ArrayExpress Uploader08593low fat dietA/Jhigh fat dietA/Jlow fat dietC57BL/6J high fat dietC57BL/6J arrayexpress_sid8The effects of high-fat (HF) feeding on the gene expression in the small intestine were examined using obesity-resistant A/J mice and...16822957E-GEOD-3433/profile/8773/arrayexpressuploader24bodyintestinelipidobesitysmall intestineDec.12, 2014Falsetranscription-profiling-of-mouse-strains-aj-versusE-GEOD-3433_A-AFFY-36Transcription profiling of mouse strains A/J versus C57BL/6J during diet-induced obesityNov.24, 2014The effects of high-fat (HF) feeding on the gene expression in the small intestine were examined using obesity-resistant A/J mice and obesity-sensitive C57BL/6J (B6) mice. Both strains of mice were maintained on low-fat (LF, 5% fat) or HF (30% fat) diets for two weeks. Oligonucleotide microarray and semi-quantitative RT-PCR analysis revealed that lipid metabolism-related genes including mitochondrial acyl-CoA thioesterase 1, pyruvate dehydrogenase kinase 4, and NADP+-dependent cytosolic malic enzyme were up-regulated by more than 2-fold in both strains of mice. The up-regulated gene expression levels were significantly higher in A/J mice than in B6 mice, suggesting the active lipid metabolism in the small intestine of A/J mice. Experiment Overall Design: Mice were maintained on the LF or HF diet for two weeks. For each group (n=6), pooled total RNA from two mice of average body weight was used for the analysis.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-3433mousehttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-3433/samples/