6ArrayExpress Uploader0mousehd/hdhd/+hd/hd+/+6708arrayexpress_sidhttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-34179We sought to identify genes regulated by the transcription factor Th-POK (Zbtb7b) in liver Va14i NKT cells, by RNA microarray analysis of...23034280/profile/8773/arrayexpressuploader14cellgenomeliverpointDec.12, 2014Falseeffect-of-th-pok-deficiency-on-global-gene-expressE-GEOD-34179_A-AFFY-45Effect of Th-POK deficiency on global gene expression in liver Va14i NKT cellsNov.12, 2014We sought to identify genes regulated by the transcription factor Th-POK (Zbtb7b) in liver Va14i NKT cells, by RNA microarray analysis of global gene expression in Va14i NKT cells from mice homozygous for the Th-POK-inactivating hd point mutation as compared with the same cell population isolated from heterozygous or wild-type age-matched mice. Two sample set pairs of hd/hd and either hd/+ or +/+ age-matched Va14i NKT cells were prepared via magnetic selection and cell sorting, and total RNA prepared using a Qiagen Rneasy mini kit. IVT probe generation and hybridization to Affymetrix Mouse Genome 430 2.0 arrays was carried out by the Veterans Medical Research Foundation GeneChipTM Microarray located at UCSD.E-GEOD-34179http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-34179/samples/