{"owner": "ArrayExpress Uploader", "ownerprofile_id": "arrayexpress_sid", "id": 6695, "factors": [{"GSM84028": {"TREATMENT": "DKK1", "CELL TYPE": "ESC-derived retinal progenitor cells"}}, {"GSM84028": {"TREATMENT": "DKK1", "CELL TYPE": "ESC-derived retinal progenitor cells"}}, {"GSM84028": {"TREATMENT": "DKK1", "CELL TYPE": "ESC-derived retinal progenitor cells"}}, {"GSM840284": {"TREATMENT": "untreated", "CELL TYPE": "ESC-derived retinal progenitor cells"}}, {"GSM840284": {"TREATMENT": "untreated", "CELL TYPE": "ESC-derived retinal progenitor cells"}}, {"GSM840284": {"TREATMENT": "untreated", "CELL TYPE": "ESC-derived retinal progenitor cells"}}, {"GSM840287": {"TREATMENT": "untreated", "CELL TYPE": "Retinal progenitor  cells from newborn C57 mice"}}, {"GSM840287": {"TREATMENT": "untreated", "CELL TYPE": "Retinal progenitor  cells from newborn C57 mice"}}, {"GSM840287": {"TREATMENT": "untreated", "CELL TYPE": "Retinal progenitor  cells from newborn C57 mice"}}], "pop_total": 0, "platform": 6, "summary_wrapped": "Tumor formation constitutes a major obstacle to the clinical application of embryonic stem cells (ESCs). As P-RPCs could successfully...", "pubmed_id": 23524971, "geo_gse_id": "E-GEOD-34002", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 2, "sample_count": 9, "tags": ["genome", "retina"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "identification-of-differential-expressed-genes-bet", "geo_id_plat": "E-GEOD-34002_A-AFFY-45", "name": "Identification of differential expressed genes between P-RPCs, ESC-RPCs and Dkk1 treated ESC-RPCs through genome-wide transcript profiling", "created": "Nov.12, 2014", "summary": "Tumor formation constitutes a major obstacle to the clinical application of embryonic stem cells (ESCs). As P-RPCs could successfully integrate into host eyes without development of teratomas or NOG, we sought to identify differentially expressed genes between P-RPCs and ESC-RPCs through genome-wide transcript profiling. Inhibition of Wnt signaling by DKK1 promotes the commitment of ESC-RPCs to more mature retinal cells and reduces the occurrence of NOG to 3%. DKK1-treated ESC-RPCs efficiently integrate to the host retina, form synaptic connections and restore visual function. Here, we report that further differentiation of ESC-derived neural progenitors into retinal progenitor cells (ESC-RPCs) completely eliminates teratomas in ocular transplantation. However, tumor-like neural overgrowth (NOG) occurs in 61% of transplanted eyes. ESC-RPCs were divided into two groups according to the differentiation stages for RNA extraction and hybridization on Affymetrix microarrays. Normal control ESC-RPCs (N) were represented the homogeneous populations of early stage expression profiles of immature ESC-RPCs. DKK1 treated ESC-RPCs (D) were represented the homogeneous populations of late stage expression profiles of further differetiated mature ESC-RPCs. To sought the pathways involved in the proliferation and oncogenesis of ESC-RPCs, the newborn C57 mice reitinal progenitor cells (R) were applied as negative control. Each group above had three independent biological repeats.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-34002", "species": "mouse", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-34002/samples/"}