ArrayExpress Uploaderarrayexpress_sidhumanwild typefemaleneuroblastoma cell lineno MYCN amplificationmutatedmaleneuroblastoma cell lineno MYCN amplificationwild typefemaleneuroblastoma cell lineno MYCN amplificationwild typemaleneuroblastoma cell lineMYCN amplifiedmutatedmaleneuroblastoma cell lineMYCN amplifiedmutatedmaleneuroblastoma cell lineMYCN amplifiedmutatedfemaleneuroblastoma cell lineMYCN amplifiedwild typemaleneuroblastoma cell lineMYCN amplifiedamplifiedfemaleneuroblastoma cell lineMYCN amplifiedmutatedfemalederivative of neuroblastoma cell line NBL16no MYCN amplificationmutatedfemalederivative of neuroblastoma cell line NBL16no MYCN amplificationwild typemaleneuroblastoma cell lineno MYCN amplificationwild typefemaleneuroblastoma cell lineMYCN amplifiedwild typemaleneuroblastoma cell lineMYCN amplifiedamplifiedfemaleneuroblastoma cell lineMYCN amplifiedmutatedfemaleneuroblastoma cell lineno MYCN amplificationamplifiedfemaleneuroblastoma cell lineMYCN amplifiednot specifiedfemalenot specifiednot specifiednot specifiedfemalenot specifiednot specifiednot specifiedfemalenot specifiednot specifiednot specifiedfemalenot specifiednot specifiednot specifiedfemalenot specifiednot specified431804To study differentially expressed genes in neuro-ectodermal cell lines MYCN amplification (NMA) is the most important prognostic factor...E-GEOD-33903/profile/8773/arrayexpressuploader422actincancercellectodermal cellneuroblastomaperipheraltyrosineDec.12, 2014FalseE-GEOD-33903_A-AFFY-44expression-array-of-peripheral-neuro-ectodermal-ceExpression array of peripheral neuro-ectodermal cell linesSep.16, 2014To study differentially expressed genes in neuro-ectodermal cell lines MYCN amplification (NMA) is the most important prognostic factor in neuroblastoma (NBL) patients, however 70% of advanced stage NBL are non-NMA and lack known driving oncogenic events. Gene expression profiles (HU133plus2.0 arrays, Affymetrix) of 17 NBL and 5 peripheral neuro-ectodermal cell lines were used to identify potential subgroups of NBL cell lines with a distinct gene signature. One group of non-NMA NBL cell lines was identified with a distinct gene expression profile and characterized by high expression of AXL. AXL is a tyrosine kinase receptor which plays a role in the metastatic process of cancer. We hypothesized that AXL contributes to the metastasizing potential of non-NMA NBL and tested if AXL silencing diminishes malignant properties of high AXL expressing cell lines. AXL was silenced in two non-NMA NBL cell lines by using a lentiviral shRNA construct that was able to transduce these cell lines with >90% infection efficiency. AXL mRNA expression level was efficiently knocked-down resulting in a severe decrease of migration of AXL positive cell lines GI-M-EN and SH-EP-2, and decreased invasion of GI-M-EN. Morphologically, AXL knockdown induced more rounded cells with a loss of contact. Intracellularly, we observed induction of stress fibers (immunofluorescence F-actin) in GI-M-EN. These changes in cytoskelet were associated with decreased migration. No effects were observed for cell proliferation, apoptosis or downstream pathways. In conclusion, AXL is identified as a possible mediator of NBL metastasis. Arrays were performed with 5 different PNET cell lines, which were used as controls for 17 NBL cell lines (GSE22771)http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-33903http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-33903/samples/