{"owner": "ArrayExpress Uploader", "ownerprofile_id": "arrayexpress_sid", "species": "human", "factors": [{"GSM563179": {"ALK MUTATION STATUS": "wild type", "GENDER": "female", "CELL TYPE": "neuroblastoma cell line", "MYCN STATUS": "no MYCN amplification"}}, {"GSM563180": {"ALK MUTATION STATUS": "mutated", "GENDER": "male", "CELL TYPE": "neuroblastoma cell line", "MYCN STATUS": "no MYCN amplification"}}, {"GSM563179": {"ALK MUTATION STATUS": "wild type", "GENDER": "female", "CELL TYPE": "neuroblastoma cell line", "MYCN STATUS": "no MYCN amplification"}}, {"GSM563182": {"ALK MUTATION STATUS": "wild type", "GENDER": "male", "CELL TYPE": "neuroblastoma cell line", "MYCN STATUS": "MYCN amplified"}}, {"GSM563183": {"ALK MUTATION STATUS": "mutated", "GENDER": "male", "CELL TYPE": "neuroblastoma cell line", "MYCN STATUS": "MYCN amplified"}}, {"GSM563183": {"ALK MUTATION STATUS": "mutated", "GENDER": "male", "CELL TYPE": "neuroblastoma cell line", "MYCN STATUS": "MYCN amplified"}}, {"GSM563185": {"ALK MUTATION STATUS": "mutated", "GENDER": "female", "CELL TYPE": "neuroblastoma cell line", "MYCN STATUS": "MYCN amplified"}}, {"GSM563182": {"ALK MUTATION STATUS": "wild type", "GENDER": "male", "CELL TYPE": "neuroblastoma cell line", "MYCN STATUS": "MYCN amplified"}}, {"GSM563187": {"ALK MUTATION STATUS": "amplified", "GENDER": "female", "CELL TYPE": "neuroblastoma cell line", "MYCN STATUS": "MYCN amplified"}}, {"GSM563188": {"ALK MUTATION STATUS": "mutated", "GENDER": "female", "CELL TYPE": "derivative of neuroblastoma cell line NBL16", "MYCN STATUS": "no MYCN amplification"}}, {"GSM563188": {"ALK MUTATION STATUS": "mutated", "GENDER": "female", "CELL TYPE": "derivative of neuroblastoma cell line NBL16", "MYCN STATUS": "no MYCN amplification"}}, {"GSM563190": {"ALK MUTATION STATUS": "wild type", "GENDER": "male", "CELL TYPE": "neuroblastoma cell line", "MYCN STATUS": "no MYCN amplification"}}, {"GSM56319": {"ALK MUTATION STATUS": "wild type", "GENDER": "female", "CELL TYPE": "neuroblastoma cell line", "MYCN STATUS": "MYCN amplified"}}, {"GSM563182": {"ALK MUTATION STATUS": "wild type", "GENDER": "male", "CELL TYPE": "neuroblastoma cell line", "MYCN STATUS": "MYCN amplified"}}, {"GSM563187": {"ALK MUTATION STATUS": "amplified", "GENDER": "female", "CELL TYPE": "neuroblastoma cell line", "MYCN STATUS": "MYCN amplified"}}, {"GSM563194": {"ALK MUTATION STATUS": "mutated", "GENDER": "female", "CELL TYPE": "neuroblastoma cell line", "MYCN STATUS": "no MYCN amplification"}}, {"GSM563187": {"ALK MUTATION STATUS": "amplified", "GENDER": "female", "CELL TYPE": "neuroblastoma cell line", "MYCN STATUS": "MYCN amplified"}}, {"GSM838617": {"ALK MUTATION STATUS": "not specified", "GENDER": "female", "CELL TYPE": "not specified", "MYCN STATUS": "not specified"}}, {"GSM838617": {"ALK MUTATION STATUS": "not specified", "GENDER": "female", "CELL TYPE": "not specified", "MYCN STATUS": "not specified"}}, {"GSM838617": {"ALK MUTATION STATUS": "not specified", "GENDER": "female", "CELL TYPE": "not specified", "MYCN STATUS": "not specified"}}, {"GSM838617": {"ALK MUTATION STATUS": "not specified", "GENDER": "female", "CELL TYPE": "not specified", "MYCN STATUS": "not specified"}}, {"GSM838617": {"ALK MUTATION STATUS": "not specified", "GENDER": "female", "CELL TYPE": "not specified", "MYCN STATUS": "not specified"}}], "id": 4318, "pop_total": 0, "platform": 4, "summary_wrapped": "To study differentially expressed genes in neuro-ectodermal cell lines MYCN amplification (NMA) is the most important prognostic factor...", "geo_gse_id": "E-GEOD-33903", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 4, "sample_count": 22, "tags": ["actin", "cancer", "cell", "ectodermal cell", "neuroblastoma", "peripheral", "tyrosine"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_id_plat": "E-GEOD-33903_A-AFFY-44", "slug": "expression-array-of-peripheral-neuro-ectodermal-ce", "geo_gds_id": "", "name": "Expression array of peripheral neuro-ectodermal cell lines", "created": "Sep.16, 2014", "summary": "To study differentially expressed genes in neuro-ectodermal cell lines MYCN amplification (NMA) is the most important prognostic factor in neuroblastoma (NBL) patients, however 70% of advanced stage NBL are non-NMA and lack known driving oncogenic events. Gene expression profiles (HU133plus2.0 arrays, Affymetrix) of 17 NBL and 5 peripheral neuro-ectodermal cell lines were used to identify potential subgroups of NBL cell lines with a distinct gene signature. One group of non-NMA NBL cell lines was identified with a distinct gene expression profile and characterized by high expression of AXL. AXL is a tyrosine kinase receptor which plays a role in the metastatic process of cancer. We hypothesized that AXL contributes to the metastasizing potential of non-NMA NBL and tested if AXL silencing diminishes malignant properties of high AXL expressing cell lines. AXL was silenced in two non-NMA NBL cell lines by using a lentiviral shRNA construct that was able to transduce these cell lines with >90% infection efficiency. AXL mRNA expression level was efficiently knocked-down resulting in a severe decrease of migration of AXL positive cell lines GI-M-EN and SH-EP-2, and decreased invasion of GI-M-EN. Morphologically, AXL knockdown induced more rounded cells with a loss of contact. Intracellularly, we observed induction of stress fibers (immunofluorescence F-actin) in GI-M-EN. These changes in cytoskelet were associated with decreased migration.  No effects were observed for cell proliferation, apoptosis or downstream pathways. In conclusion, AXL is identified as a possible mediator of NBL metastasis. Arrays were performed with 5 different PNET cell lines, which were used as controls for 17 NBL cell lines (GSE22771)", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-33903", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-33903/samples/"}