ArrayExpress Uploader02685nonetreated with scrambled siRNA, after 48hnonesiRNA trasfected - 48h of depletion from FTOnonetreated with scrambled siRNA, after 48hnonesiRNA trasfected - 48h of depletion from FTOFTO48h after induced espression of integrated copy of the FTO geneFTO24h after induced espression of integrated copy of the FTO geneFTOnoninducedFTO48h after induced espression of integrated copy of the FTO geneFTO24h after induced espression of integrated copy of the FTO geneFTOnoninducedarrayexpress_sid4To gain insight into FTO function, we knocked down and overexpressed FTO in HEK293 cells.Genetrail analyses of expression profiles...22872099E-GEOD-33870/profile/8773/arrayexpressuploader210adenineuracilDec.12, 2014Falseeffect-of-fto-overexpression-and-knockdown-on-tranE-GEOD-33870_A-AFFY-44Effect of FTO overexpression and knockdown on transcriptome of HEK293 cellsJul.12, 2014To gain insight into FTO function, we knocked down and overexpressed FTO in HEK293 cells.Genetrail analyses of expression profiles pointed to the RNA splicing and processing machinery. Intriguingly, using immunocytochemistry and confocal laser scanning microscopy, we observed strong enrichment of FTO in nuclear speckles and - to a lesser extent - in nucleoli, but not in other known nuclear bodies. We also studied RNA samples of Fto knockout and wild type mice with regard to content of methylated and unmethylated nucleosidesand observed that ratios of modified and unmodified uracil and adenine were different depending on the presence of FTO. Taken together, our data suggest that FTO is involved in RNA processing and modification. We used microarrays to investigate global gene expression changes depending on the level of FTO We compared FTO overexpressing and FTO depleted cells to cells with endogeneus level of FTO to determine global gene expression changes.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-33870humanhttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-33870/samples/