ArrayExpress Uploader0humanhuman embryonic stem cell line HSF6-8controlhuman embryonic stem cell line HSF6-8controlhuman embryonic stem cell line HSF6-8controlhuman embryonic stem cell line HSF6-8butyrate and DZNephuman embryonic stem cell line HSF6-8butyrate and DZNephuman embryonic stem cell line HSF6-8butyrate and DZNephuman embryonic stem cell line HSF6-S9butyrate and DZNephuman embryonic stem cell line HSF6-S9butyrate and DZNephuman embryonic stem cell line HSF6-S9butyrate and DZNep4316arrayexpress_sid4In order to recover nuclei with two active X chromosomes (class I), we developed a reprogramming strategy by supplementing hESC media...E-GEOD-33864/profile/8773/arrayexpressuploader29cellclassDec.12, 2014Falseeffect-of-butyrate-and-dznep-on-hesc-hsf-6E-GEOD-33864_A-AFFY-44Effect of butyrate and DZNep on hESC HSF-6Sep.16, 2014In order to recover nuclei with two active X chromosomes (class I), we developed a reprogramming strategy by supplementing hESC media with the small molecules sodium butyrate, and 3-deazaneplanocin A (DZNep). In order to determine how B+D affects global gene expression, we performed microarray analysis in triplicate in the HSF-6 (8) C and HSF-6 (8) B+D treated cultures. We also evaluated HSF-6 (S9) B+D in triplicate and identified no statistically significant changes in gene expression in HSF-6 (S9) B+D compared to HSF-6 (8) B+D treated cultures. This suggests that global transcriptional differences are more strongly modulated by presence or absence of B+D and not the percentage of class I, II or III nuclei. We performed gene expression profiling on hESC HSF-6 (8, S9) in absent (control) and presence of butyrate and DZNep. All cell were collected after 11 passages in absent and presence of butyrate and DZNep.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-33864http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-33864/samples/