{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "human", "factors": [{"GSM838174": {"CELL LINE": "human embryonic stem cell line HSF6-8", "AGENT": "control"}}, {"GSM838174": {"CELL LINE": "human embryonic stem cell line HSF6-8", "AGENT": "control"}}, {"GSM838174": {"CELL LINE": "human embryonic stem cell line HSF6-8", "AGENT": "control"}}, {"GSM838177": {"CELL LINE": "human embryonic stem cell line HSF6-8", "AGENT": "butyrate and DZNep"}}, {"GSM838177": {"CELL LINE": "human embryonic stem cell line HSF6-8", "AGENT": "butyrate and DZNep"}}, {"GSM838177": {"CELL LINE": "human embryonic stem cell line HSF6-8", "AGENT": "butyrate and DZNep"}}, {"GSM838180": {"CELL LINE": "human embryonic stem cell line HSF6-S9", "AGENT": "butyrate and DZNep"}}, {"GSM838180": {"CELL LINE": "human embryonic stem cell line HSF6-S9", "AGENT": "butyrate and DZNep"}}, {"GSM838180": {"CELL LINE": "human embryonic stem cell line HSF6-S9", "AGENT": "butyrate and DZNep"}}], "id": 4316, "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "In order to recover nuclei with two active X chromosomes (class I), we developed a reprogramming strategy by supplementing hESC media...", "geo_gse_id": "E-GEOD-33864", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 2, "sample_count": 9, "tags": ["cell", "class"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "effect-of-butyrate-and-dznep-on-hesc-hsf-6", "geo_id_plat": "E-GEOD-33864_A-AFFY-44", "name": "Effect of butyrate and DZNep on hESC HSF-6", "created": "Sep.16, 2014", "summary": "In order to recover nuclei with two active X chromosomes (class I), we developed a reprogramming strategy by supplementing hESC media with the small molecules sodium butyrate, and 3-deazaneplanocin A (DZNep).  In order to determine how B+D affects global gene expression, we performed microarray analysis in triplicate in the HSF-6 (8) C and HSF-6 (8) B+D treated cultures.  We also evaluated HSF-6 (S9) B+D in triplicate and identified no statistically significant changes in gene expression in HSF-6 (S9) B+D compared to HSF-6 (8) B+D treated cultures. This suggests that global transcriptional differences are more strongly modulated by presence or absence of B+D and not the percentage of class I, II or III nuclei. We performed gene expression profiling on hESC HSF-6 (8, S9) in absent (control) and presence of butyrate and DZNep. All cell were collected after 11 passages in absent and presence of butyrate and DZNep.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-33864", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-33864/samples/"}