{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 6679, "factors": [{"GSM829333": {"VARIATION": "Tcf1-/-"}}, {"GSM829333": {"VARIATION": "Tcf1-/-"}}, {"GSM829333": {"VARIATION": "Tcf1-/-"}}, {"GSM829336": {"VARIATION": "wild type"}}, {"GSM829336": {"VARIATION": "wild type"}}, {"GSM829336": {"VARIATION": "wild type"}}], "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "Although transcriptional programs associated with T-cell specification and commitment have been described, the functional hierarchy and...", "pubmed_id": 22109558, "geo_gse_id": "E-GEOD-33513", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 6, "tags": ["cell", "notch", "protein", "stroma", "thymocyte"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "t-cell-factor-1-is-a-gatekeeper-for-t-cell-specifi", "geo_id_plat": "E-GEOD-33513_A-AFFY-45", "name": "T cell factor 1 is a gatekeeper for T-cell specification in response to Notch signaling", "created": "Nov.12, 2014", "summary": "Although transcriptional programs associated with T-cell specification and commitment have been described, the functional hierarchy and the roles of key regulators in structuring/ orchestrating these programs remain unclear. Activation of Notch signaling in uncommitted precursors by the thymic stroma initiates the T-cell differentiation program. One regulator first induced in these precursors is the DNA binding protein Tcf-1, a T-cell specific mediator of Wnt signaling. Yet the specific contribution of Tcf-1 to early T-cell development and the signals inducing it in these cells remain unclear. Here we assign functional significance to Tcf-1 as a gatekeeper of T-cell fate. We show that Tcf-1 is directly activated by Notch signals. Tcf-1 is required at the earliest phase of Tcell determination for progression beyond the early thymic progenitor (ETP) stage. The global expression profile of Tcf-1 deficient progenitors indicates that basic processes of DNA metabolism are downregulated in its absence and the blocked T-cell progenitors become abortive and die by apoptosis. Our data thus add an important functional relationship to the roadmap of T-cell development. We used microarrays to detail the global programme of gene expression of mouse ETP thymocyte after Ikaros inactivation with dominant negative of Ik at different stage. 6 samples (mouse ETP thymocytes from wt, and Tcf1 inactivation) are analyzed Mouse Microarray Expression platforms, Affymetrix Mouse 430 2.0,", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-33513", "species": "mouse", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-33513/samples/"}