{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "human", "factors": [{"GSM828850": {"HMC1 SUBCLONE": "devoid of CD2 surface receptor expression"}}, {"GSM828849": {"HMC1 SUBCLONE": "expressing CD2 surface receptor"}}], "id": 2614, "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "Systemic mastocytosis (SM) is an incurable neoplasm characterized by abnormal accumulation of neoplastic mast cells (MC) in vascularized...", "geo_gse_id": "E-GEOD-33504", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 2, "tags": ["cell", "leukemia", "line", "mastocytosis", "organ", "p-selectin", "peritoneum", "scid", "selectin", "solid", "surface", "systemic mastocytosis"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "role-of-adhesion-receptors-in-mast-cell-disease-ev", "geo_id_plat": "E-GEOD-33504_A-AFFY-44", "name": "Role of adhesion receptors in mast cell disease-evolution", "created": "Jul.12, 2014", "summary": "Systemic mastocytosis (SM) is an incurable neoplasm characterized by abnormal accumulation of neoplastic mast cells (MC) in vascularized organs. In indolent SM, MC express several different adhesion-molecules including CD2 and CD58, and form focal tissue-aggregates, whereas in advanced SM, MC often lack CD2 and produce a more diffuse infiltration-pattern. To explore the functional role of CD2 in the pathology of SM, stable CD2+ and CD2\u2212 subclones of the human MC-leukemia cell line HMC-1 were generated and injected intraperitoneally into pfp/rag2 mice. CD2+ HMC-1 cells formed solid mastocytomas in the peritoneum and lungs, whereas CD2\u2212 cells produced diffuse infiltration. CD2+ and CD2\u2212 HMC-1 subclones all displayed the driver-mutant KIT D816V, exhibited the same growth-kinetics, and displayed identical adhesion-receptors including CD44 and selectin-ligands. To explore the mechanism of organ invasion, E- and P-selectin-deficient scid mice (scid-select) were employed. While massive HMC-1 infiltrates were detected in the lungs of control mice, infiltration was markedly reduced or absent in scid-select mice. The invasion-receptor CD44 was detectable in all MC infiltrates, with most abundant expression in the invasion-front. Together, our data show that selectins mediate organ-invasion of MC and CD2-CD58 interactions contribute to a more focal infiltration-pattern which is lost during progression to MC leukemia.  HMC1 cells were sorted by FACS for expression of CD2 surface expression. 2 subclones were obtained (CD2+ or CD2-) and compared by gene expression profiling using U133 plus 2.0 GeneChips", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-33504", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-33504/samples/"}