{"platform": 6, "owner": "ArrayExpress Uploader", "ownerprofile_id": "arrayexpress_sid", "species": "mouse", "factors": [{"GSM828146": {"VARIATION": "wild type"}}, {"GSM828146": {"VARIATION": "wild type"}}, {"GSM828146": {"VARIATION": "wild type"}}, {"GSM828149": {"VARIATION": "E\u00b5-TTP-1 transgenic"}}, {"GSM828149": {"VARIATION": "E\u00b5-TTP-1 transgenic"}}, {"GSM828149": {"VARIATION": "E\u00b5-TTP-1 transgenic"}}, {"GSM828152": {"VARIATION": "precancerous E\u03bc-Myc transgenic"}}, {"GSM828152": {"VARIATION": "precancerous E\u03bc-Myc transgenic"}}, {"GSM828152": {"VARIATION": "precancerous E\u03bc-Myc transgenic"}}, {"GSM828155": {"VARIATION": "precancerous E\u03bc-Myc;E\u00b5-TTP-1 transgenic"}}, {"GSM828155": {"VARIATION": "precancerous E\u03bc-Myc;E\u00b5-TTP-1 transgenic"}}, {"GSM828155": {"VARIATION": "precancerous E\u03bc-Myc;E\u00b5-TTP-1 transgenic"}}], "id": 6678, "pop_total": 0, "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-33474", "summary_wrapped": "Myc oncoproteins directly regulate transcription by binding to target genes, yet this only explains a fraction of the genes affected by...", "pubmed_id": 22863009, "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 12, "tags": ["bone", "bone marrow", "cancer"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "tristetraprolin-is-a-tumor-suppressor-that-impairs", "geo_id_plat": "E-GEOD-33474_A-AFFY-45", "name": "Tristetraprolin is a tumor suppressor that impairs Myc-induced lymphoma and abolishes the malignant state [bone marrow B cells].", "created": "Nov.12, 2014", "summary": "Myc oncoproteins directly regulate transcription by binding to target genes, yet this only explains a fraction of the genes affected by Myc. mRNA turnover is controlled via AU-binding proteins (AUBPs) that recognize AU-rich elements (AREs) found within many transcripts. Analyses of precancerous and malignant Myc-expressing B cells revealed that Myc regulates hundreds of ARE-containing (ARED) genes and select AUBPs. Notably, Myc directly suppresses transcription of Tristetraprolin (TTP/ZFP36), an mRNA-destabilizing AUBP, and this circuit is also operational during B lymphopoiesis and IL7 signaling. Importantly, TTP suppression is a hallmark of cancers with MYC involvement, and restoring TTP impairs Myc-induced lymphomagenesis and abolishes maintenance of the malignant state. Further, there is a selection for TTP loss in malignancy; thus, TTP functions as a tumor suppressor. Finally, Myc/TTP-directed control of select cancer-associated ARED genes is disabled during lymphomagenesis. Thus, Myc targets AUBPs to regulate ARED genes that control tumorigenesis. Wild type, E\u00b5-TTP-1, precancerous E\u03bc-Myc and precancerous E\u03bc-Myc;E\u00b5-TTP-1 transgenic bone marrow B cells (from 4-6 week old littermates; 94% C57BL/6) were investigated. These samples were used for subsequent RNA purification, labeling and hybridization to MOE430 2.0 Affymetrix arrays.", "geo_gse_id": "E-GEOD-33474", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-33474/samples/"}