{"owner": "ArrayExpress Uploader", "ownerprofile_id": "arrayexpress_sid", "species": "human", "factors": [{"GSM826709": {"TRANSFECTION": "miR-143 duplex"}}, {"GSM826709": {"TRANSFECTION": "miR-143 duplex"}}, {"GSM826709": {"TRANSFECTION": "miR-143 duplex"}}, {"GSM826709": {"TRANSFECTION": "miR-143 duplex"}}, {"GSM826713": {"TRANSFECTION": "mock"}}, {"GSM826713": {"TRANSFECTION": "mock"}}, {"GSM826713": {"TRANSFECTION": "mock"}}, {"GSM826713": {"TRANSFECTION": "mock"}}], "id": 4293, "pop_total": 0, "platform": 4, "summary_wrapped": "MicroRNAs (miRNAs) have emerged as important gene regulators and are recognized as key players in tumorigenesis. miR-143 is reported to...", "geo_gse_id": "E-GEOD-33420", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 8, "tags": ["cancer", "cell", "colon", "colon cancer", "glucose", "line"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "microrna-143-downregulates-hexokinase-2-in-colon-c", "geo_id_plat": "E-GEOD-33420_A-AFFY-44", "name": "microRNA-143 downregulates Hexokinase 2 in colon cancer cells", "created": "Sep.16, 2014", "summary": "MicroRNAs (miRNAs) have emerged as important gene regulators and are recognized as key players in tumorigenesis. miR-143 is reported to be down-regulated in several cancers, but knowledge of its targets in colon cancer remains limited. To investigate the role of miR-143 in colon cancer, we have employed a microarray based approach to identify miR-143 targets. Based on seed site enrichment analyses and unbiased word analyses, we found a significant enrichment of miRNA binding sites in the 3\u2019-untranslated regions (UTRs) of transcripts down-regulated upon miRNA overexpression. Here we identify Hexokinase 2 (HK2) as a direct target of miR-143 and show that re-introduction of miR-143 in the colon cancer cell line DLD-1 results in a decreased lactate secretion, indicating that miR-143 down-regulation of HK2 affects glucose metabolism in colon cancer cells. DLD-1 cells were transfected with 50 nM miR-143 duplex or mock transfected. Total RNA was harvested 24 hours post-transfection and analyzed on Affymetrix HG-U133 Plus 2.0 human arrays.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-33420", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-33420/samples/"}