{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSM824980": {"AGENT": "PBS"}}, {"GSM82498": {"AGENT": "MIF"}}], "id": 8584, "ownerprofile_id": "arrayexpress_sid", "platform": 8, "summary_wrapped": "Lasting B-cell persistence depends on survival signals that are transduced by cell surface receptors. Here, we describe a novel...", "geo_gse_id": "E-GEOD-33352", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 2, "tags": ["cell", "chronic lymphocytic leukemia", "cytokine", "heparin", "leukemia", "peripheral", "proteoglycan", "surface", "tyrosine"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "gene-regulation-following-mif-stimulation", "geo_id_plat": "E-GEOD-33352_A-AFFY-36", "name": "Gene regulation following MIF stimulation.", "created": "Nov.24, 2014", "summary": "Lasting B-cell persistence depends on survival signals that are transduced by cell surface receptors. Here, we describe a novel biological mechanism essential for survival and homeostasis of normal peripheral mature B cells and chronic lymphocytic leukemia (CLL) cells, regulated by the heparin-binding cytokine, midkine (MK), and its proteoglycan receptor, the receptor-type tyrosine phosphatase zeta (RPTP\u03b6). We demonstrate that MK initiates a signaling cascade leading to B cell survival, by binding to RPTP\u03b6. In mice lacking PTPRZ, the proportion and number of the mature B cell population is reduced. Our results emphasize a unique and critical function for MK signaling in the previously described MIF/CD74 induced survival pathway. Stimulation of CD74 with MIF leads to c-Met activation, resulting in elevation of MK expression in both normal mouse splenic B and CLL cells. Our results indicate that MK and RPTP\u03b6 are important regulators of the B cell repertoire. These findings could pave the way towards understanding the mechanisms shaping B cell survival, and suggest novel therapeutic strategies based on the blockade of the midkine/RPTP\u03b6-dependent survival pathway. 2 samples were incubated with or without MIF.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-33352", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-33352/samples/"}