{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "human", "factors": [{"GSM821476": {"SIRNA": "control siRNA for 48 hr"}}, {"GSM821476": {"SIRNA": "control siRNA for 48 hr"}}, {"GSM821478": {"SIRNA": "COUP-TFII siRNA for 48 hr"}}, {"GSM821478": {"SIRNA": "COUP-TFII siRNA for 48 hr"}}], "id": 4285, "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "COUP-TFII, a member of the nuclear receptor superfamily plays a critical role in angiogenesis and organogenesis during embryonic...", "geo_gse_id": "E-GEOD-33182", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 4, "tags": ["cancer", "cell", "prostate", "prostate cancer"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "expression-data-from-control-and-coup-tfii-sirna-t", "geo_id_plat": "E-GEOD-33182_A-AFFY-44", "name": "Expression data from control and COUP-TFII siRNA treated PC3 cells", "created": "Sep.16, 2014", "summary": "COUP-TFII, a member of the nuclear receptor superfamily plays a critical role in angiogenesis and organogenesis during embryonic development. Our results indicate that COUP-TFII expression is profoundly upregulated in prostate cancer patients and might serves as biomarker for recurrence prediction. Thus we conduct transcriptome comparison of control and COUP-TFII depleted PC3 cells to gain genomic insights on the biological processes that COUP-TFII is involved in prostate cancer cells. Ingenuity Pathway Analysis (IPA) shows that the most prominent altered pathways in the COUP-TFII depleted cells are related to cell growth; cell cycle progression and DNA damage response. Indeed many growth related genes including E2F1, p21, CDC25A, Cyclin A and Cyclin B are changed in COUP-TFII knockdown cells, suggesting that COUP-TFII might be an important regulator for prostate cancer cell growth. Further functional assays from cells and mice genetic studies confirm the hypothesis that COUP-TFII serve as the major regulator to control prostrate cancer growth. Together, results provide insight into the role of COUP-TFII in prostate tumorigenesis. PC3 Cells were transfected with siRNA  (Control or COUP-TFII siRNA) duplexes (40 nM) and total RNA was isolated 48 hours later.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-33182", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-33182/samples/"}