ArrayExpress Uploader0humancontrol siRNA for 48 hrcontrol siRNA for 48 hrCOUP-TFII siRNA for 48 hrCOUP-TFII siRNA for 48 hr4285arrayexpress_sid4COUP-TFII, a member of the nuclear receptor superfamily plays a critical role in angiogenesis and organogenesis during embryonic...E-GEOD-33182/profile/8773/arrayexpressuploader14cancercellprostateprostate cancerDec.12, 2014Falseexpression-data-from-control-and-coup-tfii-sirna-tE-GEOD-33182_A-AFFY-44Expression data from control and COUP-TFII siRNA treated PC3 cellsSep.16, 2014COUP-TFII, a member of the nuclear receptor superfamily plays a critical role in angiogenesis and organogenesis during embryonic development. Our results indicate that COUP-TFII expression is profoundly upregulated in prostate cancer patients and might serves as biomarker for recurrence prediction. Thus we conduct transcriptome comparison of control and COUP-TFII depleted PC3 cells to gain genomic insights on the biological processes that COUP-TFII is involved in prostate cancer cells. Ingenuity Pathway Analysis (IPA) shows that the most prominent altered pathways in the COUP-TFII depleted cells are related to cell growth; cell cycle progression and DNA damage response. Indeed many growth related genes including E2F1, p21, CDC25A, Cyclin A and Cyclin B are changed in COUP-TFII knockdown cells, suggesting that COUP-TFII might be an important regulator for prostate cancer cell growth. Further functional assays from cells and mice genetic studies confirm the hypothesis that COUP-TFII serve as the major regulator to control prostrate cancer growth. Together, results provide insight into the role of COUP-TFII in prostate tumorigenesis. PC3 Cells were transfected with siRNA (Control or COUP-TFII siRNA) duplexes (40 nM) and total RNA was isolated 48 hours later.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-33182http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-33182/samples/