ArrayExpress Uploader0humanHuman breast cancer-derived cell line HMECHuman breast cancer-derived cell line HMECHuman breast cancer-derived cell line HMECHuman breast cancer-derived cell line DKATHuman breast cancer-derived cell line DKATHuman breast cancer-derived cell line DKATHuman breast cancer-derived cell line MDA-MB-231Human breast cancer-derived cell line MDA-MB-231Human breast cancer-derived cell line MDA-MB-2314280arrayexpress_sid4The DKAT cell line is a novel model of triple-negative breast cancer that was isolated from the pleural effusion of a 35 year-old...E-GEOD-33145/profile/8773/arrayexpressuploader19breastbreast cancercancercelllineDec.12, 2014Falseexpression-data-from-dkat-and-other-breast-cancerE-GEOD-33145_A-AFFY-44Expression data from DKAT and other breast cancer cell lines under baseline growth conditionsSep.16, 2014The DKAT cell line is a novel model of triple-negative breast cancer that was isolated from the pleural effusion of a 35 year-old caucasian woman with triple-negative breast cancer. We used microarrays to look at gene expression in the DKAT cell line compared to several other commonly-used breast cancer cell lines as well as a previously published data set in order to determine the molecular subtype of the DKAT cell line. DKAT, HMEC, and MDA-MB-231 cells were cultured in Mammary Epithelial Growth Media (MEGM, Lonza). RNA was isolated using Qiagen RNeasy kit and hybridization on Affymetrix microarrays was performed. Three separate cDNA reactions were performed and these were run as replicates.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-33145http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-33145/samples/