ArrayExpress Uploaderarrayexpress_sid427204In humans, the embryonic genome activation (EGA) program is functional by day 3 after fertilization. The 6-8 cell stage embryo (day 3...22761758E-GEOD-33025/profile/8773/arrayexpressuploader06blastocystcellembryogenomeDec.12, 2014Falsecomparison-of-the-transcriptome-of-day-3-human-embE-GEOD-33025_A-AFFY-44Comparison of the transcriptome of day 3 human embryo with that of the trophectodermSep.16, 2014In humans, the embryonic genome activation (EGA) program is functional by day 3 after fertilization. The 6-8 cell stage embryo (day 3 post-fertilization) starts the process of “compaction” that leads to the generation of the tightly organized cell mass of the morula and is followed by differentiation of the morula into a blastocyst. The transition from day 3 embryos to day 5 blastocysts is likely to be controlled by many and specific changes in the expression of different genes. We used mRNA amplification technique and compared the transcriptomes of day 3 human embryos and trophectoderm (TE) cells from day 5 human blastocysts to identify transcripts that are differentially expressed during the embryo-to-TE transition and involved in the TE specification. These six embryo samples were compared to our five human trophectoderm samples that are available in GEO under the following accession numbers: GSM706168 GSM706169 GSM706170 GSM706171 GSM706172http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-33025humanhttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-33025/samples/