{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSM816995": {"PROTOCOL": "Unstimulated"}}, {"GSM816995": {"PROTOCOL": "Unstimulated"}}, {"GSM816995": {"PROTOCOL": "Unstimulated"}}, {"GSM816998": {"PROTOCOL": "GMCSF"}}, {"GSM816998": {"PROTOCOL": "GMCSF"}}, {"GSM816998": {"PROTOCOL": "GMCSF"}}, {"GSM81700": {"PROTOCOL": "Curdlan 1 \u03bcg/ml"}}, {"GSM81700": {"PROTOCOL": "Curdlan 1 \u03bcg/ml"}}, {"GSM81700": {"PROTOCOL": "Curdlan 1 \u03bcg/ml"}}, {"GSM817004": {"PROTOCOL": "Curdlan 100 \u03bcg/ml"}}, {"GSM817004": {"PROTOCOL": "Curdlan 100 \u03bcg/ml"}}, {"GSM817004": {"PROTOCOL": "Curdlan 100 \u03bcg/ml"}}, {"GSM817007": {"PROTOCOL": "Curdlan 1 \u03bcg/ml/GMCSF"}}, {"GSM817007": {"PROTOCOL": "Curdlan 1 \u03bcg/ml/GMCSF"}}, {"GSM817007": {"PROTOCOL": "Curdlan 1 \u03bcg/ml/GMCSF"}}, {"GSM817010": {"PROTOCOL": "Curdlan 100 \u03bcg/GMCSF"}}, {"GSM817010": {"PROTOCOL": "Curdlan 100 \u03bcg/GMCSF"}}, {"GSM817010": {"PROTOCOL": "Curdlan 100 \u03bcg/GMCSF"}}], "id": 6662, "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "A simultaneous engagement of different pathogen recognition receptors provides a tailor made adaptive immunity for an efficient defence...", "geo_gse_id": "E-GEOD-32986", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 18, "tags": ["chemokine", "cytokine"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "synergism-between-curdlan-and-gm-csf-in-mouse-dend", "geo_id_plat": "E-GEOD-32986_A-AFFY-45", "name": "Synergism between curdlan and GM-CSF in mouse dendritic cells", "created": "Nov.12, 2014", "summary": "A simultaneous engagement of different pathogen recognition receptors provides a tailor made adaptive immunity for an efficient defence against distinct pathogens. For example, cross talk of TLR and c-type lectin signalling effectively shapes distinct gene expression patterns by integrating the signals at the level of NF-\u03baB. Here, we extend this principle to a strong synergism between the Dectin-1 agonist, curdlan, and an inflammatory growth factor, GM-CSF. Both together act in synergy in inducing a strong inflammatory signature which converts immature DCs to potent effector DCs. A variety of cytokines (IL-1\u03b2, IL-6, TNF-\u03b1, IL-2 and IL-12p70), costimulatory molecules (CD80, CD86, CD40 and CD70), chemokines  (CxCl1, CxCl2, CxCl3, CCl12, CCl17) as well as receptors and molecules involved in fugal recognition and immunity such as Mincle, Dectin-1, Dectin-2 and Pentraxin 3 are strongly up-regulated in DC treated simultaneously with curdlan and GM-CSF. The synergistic effect of both stimuli resulted in strong IKB\u03b1 phosphorylation, in its rapid degradation and in enhanced nuclear translocation of all NF-\u03baB subunits. We further identified MAPK ERK, as one possible integration site of both signals, since its phosphorylation was clearly augmented when curdlan was co-applied with GM-CSF. Our data demonstrate that the immunomodulatory activity of curdlan requires an additional signal provided by GM-CSF to successfully initiate a robust \u03b2-glucan specific cytokine and chemokine response. The integration of both signals clearly prime and tailor a more effective innate and adaptive response against invading microbes and fungi. CD11b+ fraction of FLT3L generated BM DCs (3-4 x106) were stimulated for 4 hours with 100 or 1 \u03bcg/ml curdlan in presence or absence of 5 ng/ml GM-CSF in triplicates.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-32986", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-32986/samples/"}