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Home › Dataset Library › Dermal reprogramming through epidermal activation of beta-catenin

Dataset: Dermal reprogramming through epidermal activation of beta-catenin

Hair follicle formation depends on reciprocal epidermal-dermal interactions and occurs during skin development, but not in adult life....

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Hair follicle formation depends on reciprocal epidermal-dermal interactions and occurs during skin development, but not in adult life. This suggests that the properties of dermal fibroblasts change during postnatal development. To examine this, we used a PdgfraEGFP mouse line to isolate GFP-positive fibroblasts from neonatal skin, adult telogen and anagen skin and adult skin in which ectopic hair follicles had been induced (EF skin) by transgenic epidermal activation of beta-catenin. We also isolated epidermal cells from each mouse. The gene expression profile of EF epidermis was most similar to that of anagen epidermis, consistent with activation of beta-catenin signalling. In contrast, adult dermis with ectopic hair follicles more closely resembled neonatal dermis than adult telogen or anagen dermis. In particular, genes associated with mitosis were upregulated and extracellular matrix-associated genes were downregulated in neonatal and EF fibroblasts. We confirmed that sustained epidermal beta-catenin activation stimulated fibroblasts to proliferate to reach the high cell density of neonatal skin. In addition, the extracellular matrix was comprehensively remodelled, with mature collagen being replaced by collagen subtypes normally present only in developing skin. The changes in proliferation and extracellular matrix composition originated from a specific subpopulation of fibroblasts located beneath the sebaceous gland. Our results show that adult dermis is an unexpectedly plastic tissue that can be reprogrammed to acquire the molecular, cellular and structural characteristics of neonatal dermis in response to cues from the overlying epidermis. We have isolated the following populations of cells from mouse back skin by flow cytometry: 1A) GFP+ WT neonatal dermal fibroblasts, 1B) ItgA6+ WT neonatal epidermal keratinocytes, 2A) GFP+ WT telogen dermal fibroblasts, 2B) ItgA6+ WT telogen epidermal keratinocytes, 3A) GFP+ D2 transient activation (anagen) dermal fibroblasts, 3B) ItgA6+ D2 transient activation (anagen) epidermal keratinocytes, 4A) GFP+ D2 sustained activation (ectopic follicles) dermal fibroblasts, 4B) ItgA6+ D2 sustained activation (ectopic follicles) epidermal keratinocytes

Species:
mouse

Samples:
24

Source:
E-GEOD-32966

PubMed:
22031549

Updated:
Dec.12, 2014

Registered:
Nov.12, 2014


Factors: (via ArrayExpress)
Sample MOUSE SORTED CELL POPULATION GENOTYPE/VARIATION
GSM816522 1 GFP+ neonatal dermal fibroblasts wildtype
GSM816523 2 GFP+ neonatal dermal fibroblasts wildtype
GSM816524 3 GFP+ neonatal dermal fibroblasts wildtype
GSM816525 2 ItgA6+ neonatal epidermal keratinocytes wildtype
GSM816526 3 ItgA6+ neonatal epidermal keratinocytes wildtype
GSM816527 4 ItgA6+ neonatal epidermal keratinocytes wildtype
GSM816528 2 GFP+ telogen dermal fibroblasts wildtype
GSM816529 3 GFP+ telogen dermal fibroblasts wildtype
GSM816530 4 GFP+ telogen dermal fibroblasts wildtype
GSM81653 2 ItgA6+ telogen epidermal keratinocytes wildtype
GSM816532 3 ItgA6+ telogen epidermal keratinocytes wildtype
GSM816533 4 ItgA6+ telogen epidermal keratinocytes wildtype
GSM816534 2 GFP+ transient activated D2 dermal fibroblasts D2
GSM816535 3 GFP+ transient activated D2 dermal fibroblasts D2
GSM816536 4 GFP+ transient activated D2 dermal fibroblasts D2
GSM816537 2 ItgA6+ transient activated D2 epidermal keratinocytes D2
GSM816538 3 ItgA6+ transient activated D2 epidermal keratinocytes D2
GSM816539 4 ItgA6+ transient activated D2 epidermal keratinocytes D2
GSM816540 2 GFP+ sustained activated D2 dermal fibroblasts D2
GSM81654 3 GFP+ sustained activated D2 dermal fibroblasts D2
GSM816542 5 GFP+ sustained activated D2 dermal fibroblasts D2
GSM816543 2 ItgA6+ sustained activated D2 epidermal keratinocytes D2
GSM816544 3 ItgA6+ sustained activated D2 epidermal keratinocytes D2
GSM816545 5 ItgA6+ sustained activated D2 epidermal keratinocytes D2

Tags

  • cell
  • collagen
  • dermis
  • epidermis
  • gland
  • hair
  • hair follicle
  • line
  • sebaceous gland
  • skin

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