ArrayExpress Uploader0human4262arrayexpress_sid4Inhibiting the unfolded protein response (UPR) can be a therapeutic approach, especially for targeting the tumor microenvironment. We...E-GEOD-32911/profile/8773/arrayexpressuploader08glucoseproteinDec.12, 2014FalseE-GEOD-32911_A-AFFY-44compound-c-prevents-the-unfolded-protein-responseCompound C prevents the unfolded protein response during glucose deprivation through a mechanism independent of AMPK and BMP signaling.Sep.16, 2014Inhibiting the unfolded protein response (UPR) can be a therapeutic approach, especially for targeting the tumor microenvironment. We found that compound C (also known as dorsomorphin) prevented the UPR and exerted enhanced cytotoxicity during glucose deprivation. The UPR-inhibiting activity of compound C was not associated with either AMPK or BMP signaling inhibition. To induce the UPR, we treated HT1080 cells for 18 hours under ER stress conditions by adding 10 mM 2-Deoxy-D-glucose (2DG) to culture medium. UPR inhibitors (compound C, versipelostatin and phenformin) were added just before 2DG was added in medium. Total 8 samples were prepared for RNA extraction and hybridization on Affymetrix microarrays.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-32911http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-32911/samples/