ArrayExpress Uploader04256HMEC immortalizedexpressed hTertHMEC-derivativesexpressed hTert, Twist1 and HRasG12VHMEC-derivativesexpressed hTert, zeb1 and HRasG12VHMEC-derivativesexpressed hTert, Zeb2 and HRasG12VMCF7luminal cellMDAMB157basal B cellHMEC-derivativesexpressed hTert, Twist1 and HRasG12VHMEC-derivativesexpressed hTert, Zeb2 and HRasG12VHMEC-derivativesexpressed HRasG12VHMEC-derivativesexpressed hTert, zeb1HMEC-derivativesexpressed hTert, Zeb2HMEC-derivativesexpressed hTert, Twist1arrayexpress_sid4The newly identified claudin-low subtype of cancer is believed to represent the most primitive breast malignancies, having arisen from...22654675E-GEOD-32727/profile/8773/arrayexpressuploader212basalbreastcancercellluminalDec.12, 2014Falseemt-inducers-catalyze-malignant-transformation-ofE-GEOD-32727_A-AFFY-44EMT inducers catalyze malignant transformation of mammary epithelial cells and drive tumorigenesis towards claudin-low tumors [human]Sep.16, 2014The newly identified claudin-low subtype of cancer is believed to represent the most primitive breast malignancies, having arisen from transformation of an early epithelial precursor with inherent stemness properties and metaplastic features. Challenging this hypothesis, we show both in vitro and in vivo that transcription factors inducing epithelial-mesenchymal transition can drive the development of claudin-low tumors from differentiated mammary epithelial cells, by playing a dual role in cell transformation and dedifferentiation. Human mammary epithelial cells (HMEC) were sequentially immortalized by hTert (HMEC-hTert), transduced with Twist1 or Zeb2 or Zeb1 and then with H-RasG12V. The gene expression profiles of the resulting HMEC-hTert-Twist1+Ras, HMEC-hTert-Zeb1+Ras and HMEC-hTert-Zeb2+Ras cell lines were defined. HMEC-hTert-Twist1+Ras and HMEC-hTert-Zeb2+Ras cell lines were additionally cultured in presence of TGFÃ? and their gene expression profiles were determined. The parental HMEC-hTert, the luminal MCF7 and the basal B MDMB157cell lines were used as controls.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-32727humanhttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-32727/samples/