{"owner": "ArrayExpress Uploader", "ownerprofile_id": "arrayexpress_sid", "species": "human", "factors": [{"GSM810975 1": {}}, {"GSM810976 1": {}}, {"GSM810977 1": {}}, {"GSM810978 1": {}}, {"GSM810979 1": {}}, {"GSM810980 1": {}}, {"GSM810981 1": {}}, {"GSM810982 1": {}}], "id": 4249, "pop_total": 0, "platform": 4, "summary_wrapped": "Estrogens have been reported to activate several processes via membrane binding to either classic estrogen receptors (ERs) or GPR30. We...", "geo_gse_id": "E-GEOD-32669", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 0, "sample_count": 8, "tags": ["estrogen", "membrane"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "time-course-effect-of-estradiol-and-estradiol-bs-4", "geo_id_plat": "E-GEOD-32669_A-AFFY-44", "name": "Time-course effect of estradiol and estradiol-BSA on early gene expression in SKBR3 cells", "created": "Sep.16, 2014", "summary": "Estrogens have been reported to activate several processes via membrane binding to either classic estrogen receptors (ERs) or GPR30. We have used either estradiol or BSA-conjugated estradiol in order to initiate membrane-initiated actions and ICI 172,780 (ICI) or G15 to explore ER- and GPR30-related transcription. Our results show that the majority of G15-inhibited transcription is depending on ERs, as it is also inhibited by ICI. However, a small number of transcripts, related to specific actions/pathways is either exclusively inhibited by G15, providing evidence about a specific GPR30 signature, or not inhibited by ICI or G15 suggesting the existence of another, yet unidentified estrogen receptor. Cells after a 4h incubation with medium containing 10% charcoal stripped FBS were incubated with or without E2-BSA (10-6M) in the presence or absence of specific antagonists, in McCoys's 5A medium supplemented with 10% charcoal stripped FBS, for 3 hours. Total RNA was isolated using Nucleospin II columns (Macheray-Nagel, Dttren, Germany), according to the manufacturer\u2019s instructions. RNA was labeled and hybridized according to the Affymetrix protocol (Affymetrix Gene-Chip Expression Analysis Technical Manual), using the HGU133A plus 2 chip, analyzing a total of 54675 transcripts. Signals were detected by an Affymetrix microarray chip reader.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-32669", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-32669/samples/"}