{"platform": 3, "owner": "ArrayExpress Uploader", "ownerprofile_id": "arrayexpress_sid", "species": "human", "factors": [{"GSM807118": {"CONDITION": "BSA (control)"}}, {"GSM807118": {"CONDITION": "BSA (control)"}}, {"GSM807118": {"CONDITION": "BSA (control)"}}, {"GSM807115": {"CONDITION": "sVEGFR-1"}}, {"GSM807115": {"CONDITION": "sVEGFR-1"}}, {"GSM807115": {"CONDITION": "sVEGFR-1"}}, {"GSM807112": {"CONDITION": "fibronectin"}}, {"GSM807112": {"CONDITION": "fibronectin"}}, {"GSM807112": {"CONDITION": "fibronectin"}}], "id": 1203, "pop_total": 0, "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-32560", "summary_wrapped": "Soluble VEGFR-1 (sVEGFR-1) acts both as a decoy receptor for VEGFs and as an extracellular matrix protein for \u03b15\u03b21 integrin. A...", "pubmed_id": 24174428, "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 9, "tags": ["cell", "endothelial cell", "fibronectin", "integrin", "protein"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "svegfr-1-signaling-through-51-integrin", "geo_id_plat": "E-GEOD-32560_A-AFFY-33", "name": "sVEGFR-1 signaling through \u03b15\u03b21 integrin", "created": "Jun.18, 2014", "summary": "Soluble VEGFR-1 (sVEGFR-1) acts both as a decoy receptor for VEGFs and as an extracellular matrix protein for \u03b15\u03b21 integrin. A sVEGFR-1-derived peptide that interacts with \u03b15\u03b21 integrin promotes angiogenesis. However, canonical signal downstream integrin activation is not induced, resulting into lack of focal adhesion maturation. We performed a gene expression profile of endothelial cells adhering on sVEGFR-1 compared to that of cells adhering on fibronectin, the principal \u03b15\u03b21 integrin ligand. Three protein kinase-C substrates, adducin, MARCKS, and radixin were differently modulated. Adducin and MARCKS were less phosphorylated whereas radixin was higher phosphorylated in sVEGFR-1 adhering cells, the latter leading to prolonged small GTPase Rac1 activation and induction of a pathway involving the heterotrimeric G protein \u03b113. Altogether, our data indicated endothelial cell acquisition of an highly motile phenotype when adherent on sVEGFR-1. Finally, we indicated radixin as accountable for the angiogenic effect of \u03b15\u03b21 integrin interaction with sVEGFR-1 that in turn depends on an active VEGF-A/VEGFR-2 signaling. Endothelial cells were let adhere in Petri dishes coated with fibronectin or sVEGFR-1 before RNA extraction and hybridization on Affymetrix microarrays. Endothelial cells plated on BSA-treated Petri dishes were used as non-adhesion control. Each hybridization was performed in triplicate.", "geo_gse_id": "E-GEOD-32560", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-32560/samples/"}