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<biogps><data><item key="owner">ArrayExpress Uploader</item><item key="ownerprofile_id">arrayexpress_sid</item><item key="species">human</item><item key="factors"><item><item key="GSM804645"><item key="TRANSFECTION">shTS2</item></item></item><item><item key="GSM804645"><item key="TRANSFECTION">shTS2</item></item></item><item><item key="GSM804643"><item key="TRANSFECTION">shLuc</item></item></item><item><item key="GSM804643"><item key="TRANSFECTION">shLuc</item></item></item></item><item key="id">2274</item><item key="pop_total">0</item><item key="platform">4</item><item key="summary_wrapped">Trans-spliced isoform of NCRMS (tsNCRMS) tends to be specifically transcribed in hESCs/iPSCs and disruption of tsNCRMS expression can...</item><item key="owner_profile">/profile/8773/arrayexpressuploader</item><item key="factor_count">1</item><item key="sample_count">4</item><item key="tags"/><item key="lastmodified">Dec.12, 2014</item><item key="is_default">False</item><item key="geo_id_plat">E-GEOD-32503_A-AFFY-44</item><item key="slug">trans-splicing-in-human-embryonic-stem-cells</item><item key="source">http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-32503</item><item key="geo_gds_id"/><item key="name">Trans-splicing in human embryonic stem cells</item><item key="created">Jul.11, 2014</item><item key="summary">Trans-spliced isoform of NCRMS (tsNCRMS) tends to be specifically transcribed in hESCs/iPSCs and disruption of tsNCRMS expression can conspicuously affect the pluripotency maintenance of hESCs. We disrupt the expression of tsNCRMS by introducing tsNCRMS shTS2 that target the no-co-linear junction site in hESCs using lentiviral vectors and use shLuc as control.</item><item key="geo_gse_id">E-GEOD-32503</item><item key="sample_source">http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-32503/samples/</item></data></biogps>
