ArrayExpress Uploader0mousenot specifiedNaïve CD8 memory cellsnot specifiedNaïve CD8 memory cellsnot specifiedTim CD8 memory cellsnot specifiedTim CD8 memory cellsnot specifiedTim CD8 memory cellsnot specifiedTim CD8 memory cellsincubated with IL7, for 20h in vitroNaïve CD8 memory cells incubated with IL7, for 20h in vitroincubated with IL7, for 20h in vitroNaïve CD8 memory cells incubated with IL7, for 20h in vitroincubated with IL4 and IL7, for 20h in vitroNaïve CD8 memory cells incubated with IL4 and IL7, for 20h in vitroincubated with IL4 and IL7, for 20h in vitroNaïve CD8 memory cells incubated with IL4 and IL7, for 20h in vitroincubated with IL4 and IL7, for 20h in vitroTim CD8 memory cells incubated with IL4 and IL7, for 20h in vitroincubated with IL4 and IL7, for 20h in vitroTim CD8 memory cells incubated with IL4 and IL7, for 20h in vitroincubated with IL4 for 20h in vitroTim CD8 memory cells incubated with IL4 for 20h in vitroincubated with IL4 for 20h in vitroTim CD8 memory cells incubated with IL4 for 20h in vitroincubated with IL7 for 20h in vitroTim CD8 memory cells incubated with IL7 for 20h in vitroincubated with IL7 for 20h in vitroTim CD8 memory cells incubated with IL7 for 20h in vitroincubated 20h in vitroTim CD8 memory cells incubated 20h in vitroincubated 20h in vitroTim CD8 memory cells incubated 20h in vitro6647arrayexpress_sid6Effects of IL-4 on CD8 T cells functions are largely unknown. IL-4 induces survival and proliferation of CD8 T cells, but several studies...E-GEOD-32423/profile/8773/arrayexpressuploader218genomeDec.12, 2014Falseexpression-data-from-naive-and-memory-cd8-t-cellsE-GEOD-32423_A-AFFY-45Expression data from naive and memory CD8 T cells cultured in presence or absence of IL-4Nov.11, 2014Effects of IL-4 on CD8 T cells functions are largely unknown. IL-4 induces survival and proliferation of CD8 T cells, but several studies suggest that IL-4 could also affect several functions of CD8 T cells such as cytotoxicity. Our team has shown that IL-4 repress the expression of Ccl5 in vitro. To define more precisely the impact of IL-4 on CD8 T cells, we performed a whole genome expression microarray analysis of naive and memory CD8 T cells cultured in presence or absence of IL-4. This approach allowed us to define the IL4-gene-expression signature on CD8 T cells. 18 samples were processed. Two populations of F5 naive CD8 T cells were FACS-sorted: samples from each population were incubated 20 hours with IL-7 in presence or absence of IL-4. Thus, a total of 6 “Naive” samples were processed. In addition, 4 populations of F5 TIM memory CD8 T cells were FACS-sorted: samples from 2 of these populations were incubated 20 hours in presence of IL-7 and/or IL-4, or in medium alone. Thus, 12 “Memory” samples were processed.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-32423http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-32423/samples/