{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSM802439": {"TREATMENT": "not specified", "CELL TYPE": "Na\u00efve CD8 memory cells"}}, {"GSM802439": {"TREATMENT": "not specified", "CELL TYPE": "Na\u00efve CD8 memory cells"}}, {"GSM80244": {"TREATMENT": "not specified", "CELL TYPE": "Tim CD8 memory cells"}}, {"GSM80244": {"TREATMENT": "not specified", "CELL TYPE": "Tim CD8 memory cells"}}, {"GSM80244": {"TREATMENT": "not specified", "CELL TYPE": "Tim CD8 memory cells"}}, {"GSM80244": {"TREATMENT": "not specified", "CELL TYPE": "Tim CD8 memory cells"}}, {"GSM802445": {"TREATMENT": "incubated with IL7, for 20h in vitro", "CELL TYPE": "Na\u00efve CD8 memory cells incubated with IL7, for 20h in vitro"}}, {"GSM802445": {"TREATMENT": "incubated with IL7, for 20h in vitro", "CELL TYPE": "Na\u00efve CD8 memory cells incubated with IL7, for 20h in vitro"}}, {"GSM802447": {"TREATMENT": "incubated with IL4 and IL7, for 20h in vitro", "CELL TYPE": "Na\u00efve CD8 memory cells incubated with IL4 and IL7, for 20h in vitro"}}, {"GSM802447": {"TREATMENT": "incubated with IL4 and IL7, for 20h in vitro", "CELL TYPE": "Na\u00efve CD8 memory cells incubated with IL4 and IL7, for 20h in vitro"}}, {"GSM802449": {"TREATMENT": "incubated with IL4 and IL7, for 20h in vitro", "CELL TYPE": "Tim CD8 memory cells incubated with IL4 and IL7, for 20h in vitro"}}, {"GSM802449": {"TREATMENT": "incubated with IL4 and IL7, for 20h in vitro", "CELL TYPE": "Tim CD8 memory cells incubated with IL4 and IL7, for 20h in vitro"}}, {"GSM80245": {"TREATMENT": "incubated with IL4 for 20h in vitro", "CELL TYPE": "Tim CD8 memory cells incubated with IL4 for 20h in vitro"}}, {"GSM80245": {"TREATMENT": "incubated with IL4 for 20h in vitro", "CELL TYPE": "Tim CD8 memory cells incubated with IL4 for 20h in vitro"}}, {"GSM802453": {"TREATMENT": "incubated with IL7 for 20h in vitro", "CELL TYPE": "Tim CD8 memory cells incubated with IL7 for 20h in vitro"}}, {"GSM802453": {"TREATMENT": "incubated with IL7 for 20h in vitro", "CELL TYPE": "Tim CD8 memory cells incubated with IL7 for 20h in vitro"}}, {"GSM802455": {"TREATMENT": "incubated 20h in vitro", "CELL TYPE": "Tim CD8 memory cells incubated 20h in vitro"}}, {"GSM802455": {"TREATMENT": "incubated 20h in vitro", "CELL TYPE": "Tim CD8 memory cells incubated 20h in vitro"}}], "id": 6647, "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "Effects of IL-4 on CD8 T cells functions are largely unknown. IL-4 induces survival and proliferation of CD8 T cells, but several studies...", "geo_gse_id": "E-GEOD-32423", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 2, "sample_count": 18, "tags": ["genome"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "expression-data-from-naive-and-memory-cd8-t-cells", "geo_id_plat": "E-GEOD-32423_A-AFFY-45", "name": "Expression data from naive and memory CD8 T cells cultured in presence or absence of IL-4", "created": "Nov.11, 2014", "summary": "Effects of IL-4 on CD8 T cells functions are largely unknown. IL-4 induces survival and proliferation of CD8 T cells, but several studies suggest that IL-4 could also affect several functions of CD8 T cells such as cytotoxicity. Our team has shown that IL-4 repress the expression of Ccl5 in vitro. To define more precisely the impact of IL-4 on CD8 T cells, we performed a whole genome expression microarray analysis of naive and memory CD8 T cells cultured in presence or absence of IL-4. This approach allowed us to define the IL4-gene-expression signature on CD8 T cells. 18 samples were processed. Two populations of F5 naive CD8 T cells were FACS-sorted: samples from each population were incubated 20 hours with IL-7 in presence or absence of IL-4. Thus, a total of 6 \u201cNaive\u201d samples were processed. In addition, 4 populations of F5 TIM memory CD8 T cells were FACS-sorted: samples from 2 of these populations were incubated 20 hours in presence of IL-7 and/or IL-4, or in medium alone. Thus, 12 \u201cMemory\u201d samples were processed.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-32423", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-32423/samples/"}