ArrayExpress Uploader06624Ly6ChicontrolLy6ChicontrolLy6ChicontrolLy6ChicontrolLy6ClocontrolLy6ClocontrolLy6ClocontrolLy6ClocontrolLy6ChiRosiglitazone (Rosi)Ly6ChiRosiglitazone (Rosi)Ly6ChiRosiglitazone (Rosi)Ly6CloRosiglitazone (Rosi)Ly6CloRosiglitazone (Rosi)Ly6CloRosiglitazone (Rosi)arrayexpress_sid6PPARγ is known for its anti-inflammatory actions in macrophages. However, which macrophage populations express PPARγ in vivo and how it...22855714E-GEOD-32034/profile/8773/arrayexpressuploader214leukocytelipidlungmacrophagemonocytepulmonary alveolar proteinosisspleenDec.12, 2014Falsetissue-specific-differences-in-ppar-control-of-macE-GEOD-32034_A-AFFY-45Tissue-specific differences in PPARγ control of macrophage function.Nov.11, 2014PPARγ is known for its anti-inflammatory actions in macrophages. However, which macrophage populations express PPARγ in vivo and how it regulates tissue homeostasis in the steady state and during inflammation is not completely understood. We show that lung and spleen macrophages constitutively expressed PPARγ, while other macrophage populations did not. Recruitment of monocytes to sites of inflammation was associated with induction of PPARγ as they differentiated to macrophages. Its absence in these macrophages led to failed resolution of inflammation, characterized by persistent, low-level recruitment of leukocytes. Conversely, PPARγ agonists supported an earlier cessation in leukocyte recruitment during resolution of acute inflammation and likewise suppressed monocyte recruitment to chronically inflamed atherosclerotic vessels. In the steady state, PPARγ deficiency in macrophages had no obvious impact in the spleen but profoundly altered cellular lipid homeostasis in lung macrophages. Reminiscent of pulmonary alveolar proteinosis, LysM-Cre x PPARγflox/flox mice displayed mild leukocytic inflammation in the steady-state lung and succumbed faster to mortality upon infection with S. pneumoniae. Surprisingly, this mortality was not due to overly exuberant inflammation, but instead to impaired bacterial clearance. Thus, in addition to its anti-inflammatory role in promoting resolution of inflammation, PPARγ sustains functionality in lung macrophages and thereby has a pivotal role in supporting pulmonary host defense. The two major subsets of monocytes (Ly-6C+ and Ly-6Clo) from 12-week old C57Bl/6 mice were sorted and the RNA extracted and hybridized to Affymetrix GeneChip® 430 2.0 arrays. We pooled leukocytes from 5 mice for each sort and sorted 3 to 4 separate times for 3 to 4 biological replicates.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-32034mousehttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-32034/samples/