{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 6623, "factors": [{"GSE3203GSM71992": {"compound": "none", "genotype": "wild type", "disease": "uninfected"}}, {"GSE3203GSM71992": {"compound": "none", "genotype": "wild type", "disease": "uninfected"}}, {"GSE3203GSM71992": {"compound": "none", "genotype": "wild type", "disease": "uninfected"}}, {"GSE3203GSM71992": {"compound": "none", "genotype": "wild type", "disease": "uninfected"}}, {"GSE3203GSM71996": {"compound": "none", "genotype": "wild type", "disease": "influenza"}}, {"GSE3203GSM71996": {"compound": "none", "genotype": "wild type", "disease": "influenza"}}, {"GSE3203GSM71996": {"compound": "none", "genotype": "wild type", "disease": "influenza"}}, {"GSE3203GSM71996": {"compound": "none", "genotype": "wild type", "disease": "influenza"}}, {"GSE3203GSM72000": {"compound": "none", "genotype": "IFNR null", "disease": "influenza"}}, {"GSE3203GSM72000": {"compound": "none", "genotype": "IFNR null", "disease": "influenza"}}, {"GSE3203GSM72000": {"compound": "none", "genotype": "IFNR null", "disease": "influenza"}}, {"GSE3203GSM72000": {"compound": "none", "genotype": "IFNR null", "disease": "influenza"}}, {"GSE3203GSM72004": {"compound": "interferon beta", "genotype": "wild type", "disease": "uninfected"}}, {"GSE3203GSM72004": {"compound": "interferon beta", "genotype": "wild type", "disease": "uninfected"}}, {"GSE3203GSM72004": {"compound": "interferon beta", "genotype": "wild type", "disease": "uninfected"}}, {"GSE3203GSM72004": {"compound": "interferon beta", "genotype": "wild type", "disease": "uninfected"}}], "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "Influenza virus infection-induced gene expression changes of regional B cells are mediated at least in part through type I Interferon:;...", "pubmed_id": 17237394, "geo_gse_id": "E-GEOD-3203", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 3, "sample_count": 16, "tags": ["influenza", "lymph", "lymph node", "virus infection"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "transcription-profiling-of-mouse-influenza-virus-i", "geo_id_plat": "E-GEOD-3203_A-AFFY-45", "name": "Transcription profiling of mouse influenza virus infected in wild type and mutant animals revels induced gene expression changes of regional B cells are mediated in part through type I IFN", "created": "Nov.11, 2014", "summary": "Influenza virus infection-induced gene expression changes of regional B cells are mediated at least in part through type I Interferon:; Our objective is to determine whether the influenza virus-infection induced gene expression changes in regional lymph node B cells are facilitated at least in part through type I interferon.  Our specific aim is to compare the gene expression profile of highly FACS-purified B cells in the regional lymph nodes of wildtype and IFNR-/- mice prior to and 48h following infection with influenza virus infection and to contrast this expression profile with that of FACS-purified wildtype B cells activated in vitro with IFN-beta  anti-CD86 for 12h. Experiment Overall Design: We analyzed gene expression from mouse lymph node B cells purified by flow cytometric sorting using single channel oligonucleotide microarrays. There were 4 groups: 1) wild type uninfected mice (control group), 2) wild type mice infected with influenza (flu) for 2 days, 3) IFNR-/- mice infected with flu for 2 days, 4) cells stimulated with IFN-b in vitro for 17 h. Each group contained 4 biological replicates obtained from independent experiments. There were 16 total samples and each was measured on a separate array.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-3203", "species": "mouse", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-3203/samples/"}