{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "human", "factors": [{"GSM792049": {"DONOR": "Donor 079", "TREATMENT": "MSCs in a monolayer culture", "CELL TYPE": "synovial MSCs"}}, {"GSM792050": {"DONOR": "Donor 079", "TREATMENT": "MSC-aggregates cultured in hanging drop culture for 3 days", "CELL TYPE": "synovial MSC-aggregates"}}, {"GSM79205": {"DONOR": "Donor 083", "TREATMENT": "MSCs in a monolayer culture", "CELL TYPE": "synovial MSCs"}}, {"GSM792052": {"DONOR": "Donor 083", "TREATMENT": "MSC-aggregates cultured in hanging drop culture for 3 days", "CELL TYPE": "synovial MSC-aggregates"}}, {"GSM792053": {"DONOR": "Donor 097", "TREATMENT": "MSCs in a monolayer culture", "CELL TYPE": "synovial MSCs"}}, {"GSM792054": {"DONOR": "Donor 097", "TREATMENT": "MSC-aggregates cultured in hanging drop culture for 3 days", "CELL TYPE": "synovial MSC-aggregates"}}], "id": 4224, "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "One of strategies to regenerate cartilage defect is transplantation of mesenchymal stem cells (MSCs). Improvements of therapeutic...", "geo_gse_id": "E-GEOD-31980", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 3, "sample_count": 6, "tags": [], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "transcriptome-profile-in-the-human-synovial-msc-ag", "geo_id_plat": "E-GEOD-31980_A-AFFY-44", "name": "Transcriptome profile in the human synovial MSC-aggregates", "created": "Sep.16, 2014", "summary": "One of strategies to regenerate cartilage defect is transplantation of mesenchymal stem cells (MSCs). Improvements of therapeutic potential of MSCs are needed to achieve successful cartilage regeneration by transplantation of a limited number of cells. Aggregated culture is a popular method in ES and iPS cells to maintain or enhance their potentials. Here we investigated gene expression profile of aggregated MSCs. 621 genes were up-regulated and 409 genes were down-regulated more than 5-fold in MSC-aggregates compared with the number in MSCs in a monolayer culture. The most up-regulated gene was BMP2, which is one of the genes involved in chondrogenesis. Anti-inflammatory genes were also up-regulated in MSC-aggregates. The microarray data for selected genes were confirmed by real-time PCR. Human synovial MSCs was isolated from synovium of 3 distinct donors. The gene expression profile of MSC-aggregates cultured in hanging drop for 3days was compared with that of MSCs in a monolayer culture.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-31980", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-31980/samples/"}