ArrayExpress Uploader0humanDonor 079MSCs in a monolayer culturesynovial MSCsDonor 079MSC-aggregates cultured in hanging drop culture for 3 dayssynovial MSC-aggregatesDonor 083MSCs in a monolayer culturesynovial MSCsDonor 083MSC-aggregates cultured in hanging drop culture for 3 dayssynovial MSC-aggregatesDonor 097MSCs in a monolayer culturesynovial MSCsDonor 097MSC-aggregates cultured in hanging drop culture for 3 dayssynovial MSC-aggregates4224arrayexpress_sid4One of strategies to regenerate cartilage defect is transplantation of mesenchymal stem cells (MSCs). Improvements of therapeutic...E-GEOD-31980/profile/8773/arrayexpressuploader36Dec.12, 2014Falsetranscriptome-profile-in-the-human-synovial-msc-agE-GEOD-31980_A-AFFY-44Transcriptome profile in the human synovial MSC-aggregatesSep.16, 2014One of strategies to regenerate cartilage defect is transplantation of mesenchymal stem cells (MSCs). Improvements of therapeutic potential of MSCs are needed to achieve successful cartilage regeneration by transplantation of a limited number of cells. Aggregated culture is a popular method in ES and iPS cells to maintain or enhance their potentials. Here we investigated gene expression profile of aggregated MSCs. 621 genes were up-regulated and 409 genes were down-regulated more than 5-fold in MSC-aggregates compared with the number in MSCs in a monolayer culture. The most up-regulated gene was BMP2, which is one of the genes involved in chondrogenesis. Anti-inflammatory genes were also up-regulated in MSC-aggregates. The microarray data for selected genes were confirmed by real-time PCR. Human synovial MSCs was isolated from synovium of 3 distinct donors. The gene expression profile of MSC-aggregates cultured in hanging drop for 3days was compared with that of MSCs in a monolayer culture.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-31980http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-31980/samples/