ArrayExpress Uploaderarrayexpress_sidmouseAWTunstimulatedAMeCP2 S421AunstimulatedAWT1 hr KCl depolarizedAMeCP2 S421A1 hr KCl depolarizedAWT6 hr KCl depolarizedAMeCP2 S421A6 hr KCl depolarizedBWTunstimulatedBMeCP2 S421AunstimulatedBWT1 hr KCl depolarizedBMeCP2 S421A1 hr KCl depolarizedBWT6 hr KCl depolarizedBMeCP2 S421A6 hr KCl depolarizedCWTunstimulatedCMeCP2 S421AunstimulatedCWT1 hr KCl depolarizedCMeCP2 S421A1 hr KCl depolarizedCWT6 hr KCl depolarizedCMeCP2 S421A6 hr KCl depolarized661306To examine potential differences in activity-dependent gene expression, we analyzed mRNA expression in cultured neurons isolated from...E-GEOD-31849/profile/8773/arrayexpressuploader318leftmembraneDec.12, 2014Falsegenome-wide-activity-dependent-mecp2-phosphorylatiE-GEOD-31849_A-AFFY-45Genome-wide activity-dependent MeCP2 phosphorylation regulates nervous system development and function [cultured cortical neurons]Nov.11, 2014To examine potential differences in activity-dependent gene expression, we analyzed mRNA expression in cultured neurons isolated from Wild-type vs MeCP2 S421A mice at 0 (unstimulated), 1 or 6 hours after membrane depolarization by exposure to high extracellular KCl (55mM) All mice were male littermates from one of three litters. We isolated RNA from dissociated cortical cultures (E16+7DIV) isolated from Wild-Type or MeCP2 S421A knock-in mice littermates. Cells were either left unstimulated or depolarized for 1 or 6 hours by addition of 55mM KCl to the media. mRNA expression was analyzed using the Affymetrix GeneChip Mouse Expression Set 430 2.0 microarray platform.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-31849http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-31849/samples/