{"owner": "ArrayExpress Uploader", "ownerprofile_id": "arrayexpress_sid", "species": "mouse", "factors": [{"GSM789948": {"LITTER": "A", "GENOTYPE/VARIATION": "WT", "CONDITION": "unstimulated"}}, {"GSM789949": {"LITTER": "A", "GENOTYPE/VARIATION": "MeCP2 S421A", "CONDITION": "unstimulated"}}, {"GSM789950": {"LITTER": "A", "GENOTYPE/VARIATION": "WT", "CONDITION": "1 hr KCl depolarized"}}, {"GSM78995": {"LITTER": "A", "GENOTYPE/VARIATION": "MeCP2 S421A", "CONDITION": "1 hr KCl depolarized"}}, {"GSM789952": {"LITTER": "A", "GENOTYPE/VARIATION": "WT", "CONDITION": "6 hr KCl depolarized"}}, {"GSM789953": {"LITTER": "A", "GENOTYPE/VARIATION": "MeCP2 S421A", "CONDITION": "6 hr KCl depolarized"}}, {"GSM789954": {"LITTER": "B", "GENOTYPE/VARIATION": "WT", "CONDITION": "unstimulated"}}, {"GSM789955": {"LITTER": "B", "GENOTYPE/VARIATION": "MeCP2 S421A", "CONDITION": "unstimulated"}}, {"GSM789956": {"LITTER": "B", "GENOTYPE/VARIATION": "WT", "CONDITION": "1 hr KCl depolarized"}}, {"GSM789957": {"LITTER": "B", "GENOTYPE/VARIATION": "MeCP2 S421A", "CONDITION": "1 hr KCl depolarized"}}, {"GSM789958": {"LITTER": "B", "GENOTYPE/VARIATION": "WT", "CONDITION": "6 hr KCl depolarized"}}, {"GSM789959": {"LITTER": "B", "GENOTYPE/VARIATION": "MeCP2 S421A", "CONDITION": "6 hr KCl depolarized"}}, {"GSM789960": {"LITTER": "C", "GENOTYPE/VARIATION": "WT", "CONDITION": "unstimulated"}}, {"GSM78996": {"LITTER": "C", "GENOTYPE/VARIATION": "MeCP2 S421A", "CONDITION": "unstimulated"}}, {"GSM789962": {"LITTER": "C", "GENOTYPE/VARIATION": "WT", "CONDITION": "1 hr KCl depolarized"}}, {"GSM789963": {"LITTER": "C", "GENOTYPE/VARIATION": "MeCP2 S421A", "CONDITION": "1 hr KCl depolarized"}}, {"GSM789964": {"LITTER": "C", "GENOTYPE/VARIATION": "WT", "CONDITION": "6 hr KCl depolarized"}}, {"GSM789965": {"LITTER": "C", "GENOTYPE/VARIATION": "MeCP2 S421A", "CONDITION": "6 hr KCl depolarized"}}], "id": 6613, "pop_total": 0, "platform": 6, "summary_wrapped": "To examine potential differences in activity-dependent gene expression, we analyzed mRNA expression in cultured neurons isolated from...", "geo_gse_id": "E-GEOD-31849", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 3, "sample_count": 18, "tags": ["left", "membrane"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "genome-wide-activity-dependent-mecp2-phosphorylati", "geo_id_plat": "E-GEOD-31849_A-AFFY-45", "name": "Genome-wide activity-dependent MeCP2 phosphorylation regulates nervous system development and function [cultured cortical neurons]", "created": "Nov.11, 2014", "summary": "To examine potential differences in activity-dependent gene expression, we analyzed mRNA expression in cultured neurons isolated from Wild-type vs MeCP2 S421A mice at 0 (unstimulated), 1 or 6 hours after membrane depolarization by exposure to high extracellular KCl (55mM) All mice were male littermates from one of three litters. We isolated RNA from dissociated cortical cultures (E16+7DIV) isolated from  Wild-Type or MeCP2 S421A knock-in mice littermates. Cells were either left unstimulated or depolarized for 1 or 6 hours by addition of 55mM KCl to the media.  mRNA expression was analyzed using the Affymetrix GeneChip Mouse Expression Set 430 2.0 microarray platform.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-31849", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-31849/samples/"}