6ArrayExpress Uploaderarrayexpress_sidmouseInsig1 flox/flox /Insig2 -/- ( AT2 cells obtained from 2 months old SpC-rTTA / Otet-CRE / Insig1 flox/flox / Insig2-/- mice treated with doxycycline from E6.5 to E12.5. Control animals lacked either SpC-rtTA or Otet-CRE)Insig1 flox/flox /Insig2 -/- ( AT2 cells obtained from 2 months old SpC-rTTA / Otet-CRE / Insig1 flox/flox / Insig2-/- mice treated with doxycycline from E6.5 to E12.5. Control animals lacked either SpC-rtTA or Otet-CRE)Insig1 flox/flox /Insig2 -/- ( AT2 cells obtained from 2 months old SpC-rTTA / Otet-CRE / Insig1 flox/flox / Insig2-/- mice treated with doxycycline from E6.5 to E12.5. Control animals lacked either SpC-rtTA or Otet-CRE)Insig1 ∆/∆ /Insig2 -/- (AT2 cells obtained from 2 months old SpC-rTTA / Otet-CRE / Insig1 flox/flox / Insig2-/- mice treated with doxycycline from E6.5 to E12.5. Control animals lacked either SpC-rtTA or Otet-CRE)Insig1 ∆/∆ /Insig2 -/- (AT2 cells obtained from 2 months old SpC-rTTA / Otet-CRE / Insig1 flox/flox / Insig2-/- mice treated with doxycycline from E6.5 to E12.5. Control animals lacked either SpC-rtTA or Otet-CRE)Insig1 ∆/∆ /Insig2 -/- (AT2 cells obtained from 2 months old SpC-rTTA / Otet-CRE / Insig1 flox/flox / Insig2-/- mice treated with doxycycline from E6.5 to E12.5. Control animals lacked either SpC-rtTA or Otet-CRE)66110http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-31797Background: Lung function is dependent upon the precise regulation of the synthesis, storage, and catabolism of tissue and alveolar.../profile/8773/arrayexpressuploader16genomelipidlungobesityproteinDec.12, 2014Falseactivation-of-srebp-in-alveolar-type-ii-cells-enhaE-GEOD-31797_A-AFFY-45Activation of SREBP in Alveolar Type II Cells Enhances Lipogenesis Causing Pulmonary LipotoxicityNov.11, 2014Background: Lung function is dependent upon the precise regulation of the synthesis, storage, and catabolism of tissue and alveolar lipids. Results: Activation of SREBP (Sterol Response Element Binding Protein) induced lipogenesis in alveolar epithelial cells, causing neutral lipid accumulation, lung inflammation, and tissue remodeling. Conclusions: The accumulation of neutral lipids in type II epithelial cells and alveolar macrophages caused lung inflammation, consistent with findings in lipid storage disorders. Significance: Pulmonary lipotoxicity may contribute to the pathogenesis of lung dysfunction associated with diabetes, obesity, and other metabolic disorders. Genome-wide transcription profiling comparison between doxycycline-exposed SFTPC-rtTAWT/Tg/(tetO)7CMV-CreWT/Tg/Insig1flox/flox/Insig2-/- mice (i.e., Insig1/2∆/∆ ) and Insig1flox/flox/Insig2-/- . Three independent pooled RNA from isolated lung type 2 cells of each genotype were used.E-GEOD-31797http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-31797/samples/