ArrayExpress Uploader0humanBecotide, Ventolin9882male223Non_severe_1CD4+ T-cellsflixotide9287female244Non_severe_2CD4+ T-cellsBecotide, Ventolin8977male235Non_severe_3CD4+ T-cellsSalbutamol8184female223Non_severe_4CD4+ T-cellsNone7678male139Control_1CD4+ T-cellsNone107103female125Control_2CD4+ T-cellsNone115105female126Control_3CD4+ T-cellsNone10592male146Control_4CD4+ T-cellsNone7778male133Control_5CD4+ T-cellsNone115116female133Control_6CD4+ T-cellsNone9685female131Control_7CD4+ T-cellsNone111111male156Control_8CD4+ T-cellsFlixotide 500mcg 2pbd,Oxis 12 1pbd,Loratadine 1 tab od,Beconase 2pbd10582female155Severe_1CD4+ T-cellsPrednisolone 8mg - 1mg for 5wks(stopped 30/5/08),seretide 500mcg 1pbd,Singular 10mg bd,Phyllocontin 225mg am 450mg pm,Ventolin prn101105female121Severe_2CD4+ T-cellsprednisolone 15mg od,Seretide 500mcg 2pbd,Montelukast 10mg nocte,Ventolin prn,Phyllocontin 450mg bd,Flixonase od11398male132Severe_3CD4+ T-cellsPrednisolone 10mg od,Spiriva 18mcg od,Methotrexate 12.5mg wkly, Seretode 500 1pbd,Fosamax 70mg once/wk,Folic acid 5mg od,Calchichew,Cocodamol prn8165female155Severe_4CD4+ T-cellsPrednisolone 5mg od,Montelukast 10mg od,Symbicort 400/12 2pbd,Omeprazole 20mg od,Uniphyllin 400mg bd,Bricanyl prn6144female154Severe_5CD4+ T-cellsPrednisolone 10-20mg od,Combivent neb 2-4/day,Cetirizine 10mg od,Terbutaline 500mcg od,Budesonide 400mcg 2pbd, Aminophylline SR 450mg bd, Formoterol 12mcg 2pbd6955female154Severe_6CD4+ T-cellsPrednisolone 10mg od, Insulin S/C,Seretide 500mcg 2pbd, Metformin,Nexium, Flixonase,Tramacet,Calcium,Valsartan,Amitriptyline10571female145Severe_7CD4+ T-cellsPrednisolone 5mg od, Aminophylline 225mg bd,Seretide 500 2pbd,doxycycline, Tiotropium9433female142Severe_8CD4+ T-cellsBecotide, Ventolin9882male223Non_severe_1CD8+ T-cellsflixotide9287female244Non_severe_2CD8+ T-cellsBecotide, Ventolin8977male235Non_severe_3CD8+ T-cellsSalbutamol8184female223Non_severe_4CD8+ T-cellsFlixotide 500mcg 2pbd,Oxis 12 1pbd,Loratadine 1 tab od,Beconase 2pbd10582female155Severe_1CD8+ T-cellsPrednisolone 8mg - 1mg for 5wks(stopped 30/5/08),seretide 500mcg 1pbd,Singular 10mg bd,Phyllocontin 225mg am 450mg pm,Ventolin prn101105female121Severe_2CD8+ T-cellsprednisolone 15mg od,Seretide 500mcg 2pbd,Montelukast 10mg nocte,Ventolin prn,Phyllocontin 450mg bd,Flixonase od11398male132Severe_3CD8+ T-cellsPrednisolone 10mg od,Spiriva 18mcg od,Methotrexate 12.5mg wkly, Seretode 500 1pbd,Fosamax 70mg once/wk,Folic acid 5mg od,Calchichew,Cocodamol prn8165female155Severe_4CD8+ T-cellsPrednisolone 5mg od,Montelukast 10mg od,Symbicort 400/12 2pbd,Omeprazole 20mg od,Uniphyllin 400mg bd,Bricanyl prn6144female154Severe_5CD8+ T-cellsPrednisolone 10-20mg od,Combivent neb 2-4/day,Cetirizine 10mg od,Terbutaline 500mcg od,Budesonide 400mcg 2pbd, Aminophylline SR 450mg bd, Formoterol 12mcg 2pbd6955female154Severe_6CD8+ T-cellsPrednisolone 10mg od, Insulin S/C,Seretide 500mcg 2pbd, Metformin,Nexium, Flixonase,Tramacet,Calcium,Valsartan,Amitriptyline10571female145Severe_7CD8+ T-cellsPrednisolone 5mg od, Aminophylline 225mg bd,Seretide 500 2pbd,doxycycline, Tiotropium9433female142Severe_8CD8+ T-cellsNone7678male139Control_1CD8+ T-cellsNone107103female125Control_2CD8+ T-cellsNone115105female126Control_3CD8+ T-cellsNone10592male146Control_4CD8+ T-cellsNone7778male133Control_5CD8+ T-cellsNone115116female133Control_6CD8+ T-cellsNone9685female131Control_7CD8+ T-cellsNone111111male156Control_8CD8+ T-cells4220arrayexpress_sid4Comparison of mRNA expression showed widespread changes in the circulating CD8+ but not CD4+ T-cells from patients with severe asthma. No...E-GEOD-31773/profile/8773/arrayexpressuploader840asthmacellDec.12, 2014Falsecomparison-of-mrna-expression-in-circulating-t-celE-GEOD-31773_A-AFFY-44Comparison of mRNA expression in circulating T-cells from patients with severe asthmaSep.16, 2014Comparison of mRNA expression showed widespread changes in the circulating CD8+ but not CD4+ T-cells from patients with severe asthma. No changes were observed in the CD4+ and CD8+ T-cells in non-severe asthmatics versus healthy controls. Bioinformatics analysis showed that the changes in CD8+ T-cell mRNA expression were associated with multiple pathways involved in T-cell activation. As with mRNAs, we also observed widespread changes in expression of non-coding RNA species including natural antisense, pseudogenes, intronic long ncRNAs and long intergenic long ncRNAs in CD8+ T-cells from severe asthmatics. Measurement of the miRNA expression profile showed selective down-regulation of miR-28-5p in CD8+ T-cells and reduction of miR-146a and miR-146b in both CD4+ and CD8+ T-cells. mRNA samples were collected from circulating CD4+ and CD8+ T-cells from healthy donors as well as donors with severe and non-severe asthma.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-31773http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-31773/samples/