Dataset: Comparison of mRNA expression in circulating T-cells from patients with severe asthma

Comparison of mRNA expression showed widespread changes in the circulating CD8+ but not CD4+ T-cells from patients with severe asthma. No...

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Comparison of mRNA expression showed widespread changes in the circulating CD8+ but not CD4+ T-cells from patients with severe asthma. No changes were observed in the CD4+ and CD8+ T-cells in non-severe asthmatics versus healthy controls. Bioinformatics analysis showed that the changes in CD8+ T-cell mRNA expression were associated with multiple pathways involved in T-cell activation. As with mRNAs, we also observed widespread changes in expression of non-coding RNA species including natural antisense, pseudogenes, intronic long ncRNAs and long intergenic long ncRNAs in CD8+ T-cells from severe asthmatics. Measurement of the miRNA expression profile showed selective down-regulation of miR-28-5p in CD8+ T-cells and reduction of miR-146a and miR-146b in both CD4+ and CD8+ T-cells. mRNA samples were collected from circulating CD4+ and CD8+ T-cells from healthy donors as well as donors with severe and non-severe asthma.

Species:: human
Samples:: 40
Source:: E-GEOD-31773
Updated:: Dec.12, 2014
Registered:: Sep.16, 2014

Factors: (via ArrayExpress)

Sample	MEDICATION	FVC (% PREDICTED)	FEV1 (% PREDICTED)	GENDER	BATCH	AGE (YEARS)	PATIENT ID	CELL TYPE
GSM788299	Becotide, Ventolin	98	82	male	2	23	Non_severe_1	CD4+ T-cells
GSM788300	flixotide	92	87	female	2	44	Non_severe_2	CD4+ T-cells
GSM78830	Becotide, Ventolin	89	77	male	2	35	Non_severe_3	CD4+ T-cells
GSM788302	Salbutamol	81	84	female	2	23	Non_severe_4	CD4+ T-cells
GSM788303	None	76	78	male	1	39	Control_1	CD4+ T-cells
GSM788304	None	107	103	female	1	25	Control_2	CD4+ T-cells
GSM788305	None	115	105	female	1	26	Control_3	CD4+ T-cells
GSM788306	None	105	92	male	1	46	Control_4	CD4+ T-cells
GSM788307	None	77	78	male	1	33	Control_5	CD4+ T-cells
GSM788308	None	115	116	female	1	33	Control_6	CD4+ T-cells
GSM788309	None	96	85	female	1	31	Control_7	CD4+ T-cells
GSM788310	None	111	111	male	1	56	Control_8	CD4+ T-cells
GSM7883	Flixotide 500mcg 2pbd,Oxis 12 1pbd,Loratadine 1 tab od,Beconase 2pbd	105	82	female	1	55	Severe_1	CD4+ T-cells
GSM788312	Prednisolone 8mg - 1mg for 5wks(stopped 30/5/08),seretide 500mcg 1pbd,Singular 10mg bd,Phyllocontin 225mg am 450mg pm,Ventolin prn	101	105	female	1	21	Severe_2	CD4+ T-cells
GSM788313	prednisolone 15mg od,Seretide 500mcg 2pbd,Montelukast 10mg nocte,Ventolin prn,Phyllocontin 450mg bd,Flixonase od	113	98	male	1	32	Severe_3	CD4+ T-cells
GSM788314	Prednisolone 10mg od,Spiriva 18mcg od,Methotrexate 12.5mg wkly, Seretode 500 1pbd,Fosamax 70mg once/wk,Folic acid 5mg od,Calchichew,Cocodamol prn	81	65	female	1	55	Severe_4	CD4+ T-cells
GSM788315	Prednisolone 5mg od,Montelukast 10mg od,Symbicort 400/12 2pbd,Omeprazole 20mg od,Uniphyllin 400mg bd,Bricanyl prn	61	44	female	1	54	Severe_5	CD4+ T-cells
GSM788316	Prednisolone 10-20mg od,Combivent neb 2-4/day,Cetirizine 10mg od,Terbutaline 500mcg od,Budesonide 400mcg 2pbd, Aminophylline SR 450mg bd, Formoterol 12mcg 2pbd	69	55	female	1	54	Severe_6	CD4+ T-cells
GSM788317	Prednisolone 10mg od, Insulin S/C,Seretide 500mcg 2pbd, Metformin,Nexium, Flixonase,Tramacet,Calcium,Valsartan,Amitriptyline	105	71	female	1	45	Severe_7	CD4+ T-cells
GSM788318	Prednisolone 5mg od, Aminophylline 225mg bd,Seretide 500 2pbd,doxycycline, Tiotropium	94	33	female	1	42	Severe_8	CD4+ T-cells
GSM788319	Becotide, Ventolin	98	82	male	2	23	Non_severe_1	CD8+ T-cells
GSM788320	flixotide	92	87	female	2	44	Non_severe_2	CD8+ T-cells
GSM78832	Becotide, Ventolin	89	77	male	2	35	Non_severe_3	CD8+ T-cells
GSM788322	Salbutamol	81	84	female	2	23	Non_severe_4	CD8+ T-cells
GSM788323	Flixotide 500mcg 2pbd,Oxis 12 1pbd,Loratadine 1 tab od,Beconase 2pbd	105	82	female	1	55	Severe_1	CD8+ T-cells
GSM788324	Prednisolone 8mg - 1mg for 5wks(stopped 30/5/08),seretide 500mcg 1pbd,Singular 10mg bd,Phyllocontin 225mg am 450mg pm,Ventolin prn	101	105	female	1	21	Severe_2	CD8+ T-cells
GSM788325	prednisolone 15mg od,Seretide 500mcg 2pbd,Montelukast 10mg nocte,Ventolin prn,Phyllocontin 450mg bd,Flixonase od	113	98	male	1	32	Severe_3	CD8+ T-cells
GSM788326	Prednisolone 10mg od,Spiriva 18mcg od,Methotrexate 12.5mg wkly, Seretode 500 1pbd,Fosamax 70mg once/wk,Folic acid 5mg od,Calchichew,Cocodamol prn	81	65	female	1	55	Severe_4	CD8+ T-cells
GSM788327	Prednisolone 5mg od,Montelukast 10mg od,Symbicort 400/12 2pbd,Omeprazole 20mg od,Uniphyllin 400mg bd,Bricanyl prn	61	44	female	1	54	Severe_5	CD8+ T-cells
GSM788328	Prednisolone 10-20mg od,Combivent neb 2-4/day,Cetirizine 10mg od,Terbutaline 500mcg od,Budesonide 400mcg 2pbd, Aminophylline SR 450mg bd, Formoterol 12mcg 2pbd	69	55	female	1	54	Severe_6	CD8+ T-cells
GSM788329	Prednisolone 10mg od, Insulin S/C,Seretide 500mcg 2pbd, Metformin,Nexium, Flixonase,Tramacet,Calcium,Valsartan,Amitriptyline	105	71	female	1	45	Severe_7	CD8+ T-cells
GSM788330	Prednisolone 5mg od, Aminophylline 225mg bd,Seretide 500 2pbd,doxycycline, Tiotropium	94	33	female	1	42	Severe_8	CD8+ T-cells
GSM78833	None	76	78	male	1	39	Control_1	CD8+ T-cells
GSM788332	None	107	103	female	1	25	Control_2	CD8+ T-cells
GSM788333	None	115	105	female	1	26	Control_3	CD8+ T-cells
GSM788334	None	105	92	male	1	46	Control_4	CD8+ T-cells
GSM788335	None	77	78	male	1	33	Control_5	CD8+ T-cells
GSM788336	None	115	116	female	1	33	Control_6	CD8+ T-cells
GSM788337	None	96	85	female	1	31	Control_7	CD8+ T-cells
GSM788338	None	111	111	male	1	56	Control_8	CD8+ T-cells