{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "human", "factors": [{"GSM780873 1": {}}, {"GSM780874 1": {}}, {"GSM780875 1": {}}, {"GSM780876 1": {}}], "id": 4205, "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "Colon cancer cell line RKO grown on Matrigel form a luminal-like structure after the transfection of SPARCL1 gene, which may imply the...", "geo_gse_id": "E-GEOD-31440", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 0, "sample_count": 4, "tags": ["cancer", "cell", "colon", "colon cancer", "dish", "line", "luminal"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "expression-data-from-rko-cells-transfected-with-sp", "geo_id_plat": "E-GEOD-31440_A-AFFY-44", "name": "Expression data from RKO cells transfected with SPARCL1 and vector control", "created": "Sep.16, 2014", "summary": "Colon cancer cell line RKO grown on Matrigel form a luminal-like structure after the transfection of SPARCL1 gene, which may imply the differentiation of the cells. To indentify genes regulated by SPARCL1, we built two stable cell lines: RKO with pLXSN-SPARCL1 plasmid (RKO-SPARCL1) and RKO with pLXSN plasmid (RKO-pLXSN). Then we cultured these two cell lines on both plastic dish and dish cover with a layer of Matrigel. We used microarrays to undentify global gene expression change in RKO cells after the tranfection of SPARCL1. Four samples including RKO-pLXSN cells cultured on plastic, RKO-pLXSN cells cultured on Matrigel, RKO-SPARCL1 cells cultured on plastic and RKO-SPARCL1 cells cultured on Matrigel were trypsinized and collected for RNA extraction and hybridization on Affymetrix microarray U133plus2.0. Supplementary files: * RLvsRP: up and down regulated genes comparing with RKO-SPARCL1(RL) and RKO-pLXSN(RP) cells cultured on plastic * RLMvsRPM: up and down regulated genes comparing with RKO-SPARCL1(RL) and RKO-pLXSN(RP) cells cultured on Matrigel", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-31440", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-31440/samples/"}