ArrayExpress Uploader0humanmacroscopically unaffected fascia tissue from the edge of primary incision (ND)palmar fascia localized in palmodigital and digital area (D)macroscopically unaffected fascia tissue from the edge of primary incision (ND)palmar fascia localized in palmodigital and digital area (D)macroscopically unaffected fascia tissue from the edge of primary incision (ND)palmar fascia localized in palmodigital and digital area (D)1286arrayexpress_sid3We used a high-throughput technology, DNA microarray, to screen the entire genome for the changes in gene expression in diseased tissue...E-GEOD-31356/profile/8773/arrayexpressuploader16celldiseasegenomeDec.12, 2014Falsemicroarray-expression-analysis-of-patients-dupuytrE-GEOD-31356_A-AFFY-33Microarray expression analysis of patients Dupuytren´s contracture (DC)Jun.18, 2014We used a high-throughput technology, DNA microarray, to screen the entire genome for the changes in gene expression in diseased tissue to characterize Dupuytren's contracture at a molecular level and find genes that are involved in development of the disease. Primary cell cultures were established from surgically removed diseased (D) and healthy (ND) tissue from 3 patients. Total we had 6 samples, 3 diseased and 3 healthy patient-matched samples. Total RNA was isolated by RNeasy mini kit (Qiagen) and further used for microarray expression analysis (HG-U133A array, Affymetrix). Raw data, CEL. files were analysed with software MAS5, GeneSpring and GENEMAPP.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-31356http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-31356/samples/