ArrayExpress Uploader041951EPHB2 high1EPHB2 medium1EPHB2 low1EPHB2 neg2EPHB2 high2EPHB2 medium2EPHB2 low2EPHB2 neg3EPHB2 high3EPHB2 medium3EPHB2 lowarrayexpress_sid4Using the surface marker EPHB2, we have FACS-purified and profiled stem cell-enriched cell fractions from normal human mucosa, crypt...21892181E-GEOD-31255/profile/8773/arrayexpressuploader211cellcolonmucosastem cellsurfaceDec.12, 2014Falseisolation-and-in-vitro-expansion-of-human-colonicE-GEOD-31255_A-AFFY-44Isolation and in vitro expansion of human colonic stem cells [Expression profile]Sep.16, 2014Using the surface marker EPHB2, we have FACS-purified and profiled stem cell-enriched cell fractions from normal human mucosa, crypt proliferative progenitors and late transient amplifying cells to define a gene expression program specific for normal human colon epithelial stem cells We FACS purified human colonic crypt cells according to their EPHB2 surface abundance. We used Affymetrix chips to hybridize 3 samples from EPHB2-high, 3 samples from EPHB2-medium, 3 samples from EPHB2-low, and 2 samples from EPHB2-negative cellshttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-31255humanhttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-31255/samples/