Dataset: Isolation and in vitro expansion of human colonic stem cells [Expression profile]
Using the surface marker EPHB2, we have FACS-purified and profiled stem cell-enriched cell fractions from normal human mucosa, crypt...
Using the surface marker EPHB2, we have FACS-purified and profiled stem cell-enriched cell fractions from normal human mucosa, crypt proliferative progenitors and late transient amplifying cells to define a gene expression program specific for normal human colon epithelial stem cells We FACS purified human colonic crypt cells according to their EPHB2 surface abundance. We used Affymetrix chips to hybridize 3 samples from EPHB2-high, 3 samples from EPHB2-medium, 3 samples from EPHB2-low, and 2 samples from EPHB2-negative cells
- Species:
- human
- Samples:
- 11
- Source:
- E-GEOD-31255
- PubMed:
- 21892181
- Updated:
- Dec.12, 2014
- Registered:
- Sep.16, 2014
Factors:
(via ArrayExpress)
| Sample | PATIENT ID | EPHB2 SURFACE ABUNDANCE |
|---|---|---|
| GSM774695 | 1 | EPHB2 high |
| GSM774696 | 1 | EPHB2 medium |
| GSM774697 | 1 | EPHB2 low |
| GSM774698 | 1 | EPHB2 neg |
| GSM774699 | 2 | EPHB2 high |
| GSM774700 | 2 | EPHB2 medium |
| GSM77470 | 2 | EPHB2 low |
| GSM774702 | 2 | EPHB2 neg |
| GSM774703 | 3 | EPHB2 high |
| GSM774704 | 3 | EPHB2 medium |
| GSM774705 | 3 | EPHB2 low |