6ArrayExpress Uploader06573arrayexpress_sidhttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-31165In order to identify the effects of the knock-down of the gene of interest on the mouse ES transcriptome, we performed Affymetrix Gene-...23180766/profile/8773/arrayexpressuploader05celllineDec.12, 2014Falseexpression-data-from-knock-down-es-stable-cell-linE-GEOD-31165_A-AFFY-45Expression data from knock-down ES stable cell lineNov.11, 2014In order to identify the effects of the knock-down of the gene of interest on the mouse ES transcriptome, we performed Affymetrix Gene-Chip hybridization experiments for the knock-down cell line. Transcriptome analysis of the knock-down transgenic mouse ES cell line. The knock-down cell line (shE13) was generated by stably expressing a specific short-hairpin RNA against E13 sequence thus knocking-down E13 expression in parental mouse ES cell line E14Tg2a.4 (E14, Hooper M et al., 1987). The specific mouse gene knocked down in the ES cell line is E130012A19Rik. For the analysis on knock-down cell line, total RNA extracted from three different shE13 clones was compared to total RNA extracted from two shCTL clonesE-GEOD-31165mousehttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-31165/samples/