{"platform": 6, "owner": "ArrayExpress Uploader", "pop_total": 0, "id": 6573, "factors": [{"GSM772499 1": {}}, {"GSM772500 1": {}}, {"GSM772501 1": {}}, {"GSM772502 1": {}}, {"GSM772503 1": {}}], "ownerprofile_id": "arrayexpress_sid", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-31165", "summary_wrapped": "In order to identify the effects of the knock-down of the gene of interest on the mouse ES transcriptome, we performed Affymetrix Gene-...", "pubmed_id": 23180766, "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 0, "sample_count": 5, "tags": ["cell", "line"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "expression-data-from-knock-down-es-stable-cell-lin", "geo_id_plat": "E-GEOD-31165_A-AFFY-45", "name": "Expression data from knock-down ES stable cell line", "created": "Nov.11, 2014", "summary": "In order to identify the effects of the knock-down of the gene of interest on the mouse ES transcriptome, we performed Affymetrix Gene-Chip hybridization experiments for the knock-down cell line. Transcriptome analysis of the knock-down transgenic mouse ES cell line. The knock-down cell line (shE13) was generated by stably expressing a specific short-hairpin RNA against E13 sequence thus knocking-down E13 expression in parental mouse ES cell line E14Tg2a.4 (E14, Hooper M et al., 1987). The specific mouse gene knocked down in the ES cell line is E130012A19Rik. For the analysis on knock-down cell line, total RNA extracted from three different shE13 clones was compared to total RNA extracted from two shCTL clones", "geo_gse_id": "E-GEOD-31165", "species": "mouse", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-31165/samples/"}