8ArrayExpress Uploader0mouseaTC1DMSOaTC1DMSOaTC1DMSOaTC1GW8510aTC1GW8510aTC1GW8510bTC3DMSObTC3DMSObTC3DMSObTC3GW8510bTC3GW8510bTC3GW85108569arrayexpress_sidhttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-31102Expression of insulin in terminally differentiated non-beta pancreatic cell types could be important for treating type-1 diabetes. We.../profile/8773/arrayexpressuploader212cellinsulinlineDec.12, 2014Falseexpression-data-from-gw8510-treatment-of-pancreatiE-GEOD-31102_A-AFFY-36Expression data from GW8510 treatment of pancreatic cellsNov.24, 2014Expression of insulin in terminally differentiated non-beta pancreatic cell types could be important for treating type-1 diabetes. We observed that the kinase inhibitor GW8510 up-regulated insulin expression in mouse pancreatic alpha cells. Gene-expression profiling and gene-set enrichment analysis of GW8510-treated alpha cells suggested coordinate up-regulation of the p53 pathway, which was further implicated in induction of insulin transcript. In order to determine a potential mechanism of insulin induction by GW8510, we treated mouse alpha cells (aTC1) and beta cells (bTC3) with 3.3 µM GW8510 or 0.1% DMSO for five days, and performed gene-expression profiling followed by gene-set enrichment analysis (GSEA). Three biological replicates were included per condition. GW8510 treatment in each cell line was compared to DMSO-treated control.E-GEOD-31102http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-31102/samples/