Dataset: Human Natural Killer Cell activation by mycobacteria
Purified NK cells were co-cultured with M. bovis BCG or M. tuberculosis H37Rv (1:1) in the presence of IL-2 (100U/ml) or IL-12 (10pg/ml)...
Purified NK cells were co-cultured with M. bovis BCG or M. tuberculosis H37Rv (1:1) in the presence of IL-2 (100U/ml) or IL-12 (10pg/ml) for 24h before trizol extraction. We used microarrays to detail the global gene expression underlying NK cell activation by mycobacteria. NK cell were isolated from the blood of 6 independent donors and activated with different mycobacteria and cytokines in order to study their transcriptional profiles according to mycobacterial virulence.
- Species:
- human
- Samples:
- 18
- Source:
- E-GEOD-30950
- Updated:
- Dec.12, 2014
- Registered:
- Sep.16, 2014
Factors:
(via ArrayExpress)
| Sample | DONOR | TREATMENT |
|---|---|---|
| GSM767357 | F2 | IL-12 alone |
| GSM767358 | F2 | IL-12 + BCG |
| GSM767359 | F2 | IL-12 + H37Rv |
| GSM767360 | G2 | IL-12 alone |
| GSM76736 | G2 | IL-12 + BCG |
| GSM767362 | G2 | IL-12 + H37Rv |
| GSM767363 | H2 | IL-12 alone |
| GSM767364 | H2 | IL-12 + BCG |
| GSM767365 | H2 | IL-12 + H37Rv |
| GSM767366 | I2 | IL-12 alone |
| GSM767367 | I2 | IL-12 + BCG |
| GSM767368 | I2 | IL-12 + H37Rv |
| GSM767369 | J2 | IL-2 alone |
| GSM767370 | J2 | IL-2 + BCG |
| GSM76737 | J2 | IL-2 + H37Rv |
| GSM767372 | K2 | IL-2 alone |
| GSM767373 | K2 | IL-2 + BCG |
| GSM767374 | K2 | IL-2 + H37Rv |