{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "human", "factors": [{"GSM76420": {"CELL TYPE": "osteosarcoma U2OS derived cell line UT2"}}, {"GSM764200": {"CELL TYPE": "osteosarcoma U2OS cells"}}, {"GSM764199": {"CELL TYPE": "bone marrow mesenchymal stem cells"}}], "id": 2628, "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "To determine whether enhanced self-renewal and tumorigenicity in UT2 cells (derived from the second human\u2013mouse xenotransplantation of...", "geo_gse_id": "E-GEOD-30807", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 3, "tags": ["cancer", "cell", "notch", "osteosarcoma"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_id_plat": "E-GEOD-30807_A-AFFY-44", "slug": "expression-data-from-human-mesenchymal-stem-cell-a", "geo_gds_id": "", "name": "Expression data from human mesenchymal stem cell and osteosarcoma cells", "created": "Jul.12, 2014", "summary": "To determine whether enhanced self-renewal and tumorigenicity in UT2 cells (derived from the second human\u2013mouse xenotransplantation of U2OS cell-formed osteosarcoma tissues) correlate with increased expression of stem/progenitor cell-associated genes, we measured global gene expression in MSC, U2OS and UT2 cells by microarray analysis. Compared to U2OS and MSC, molecules involved in regulation of self-renewal signaling pathways of cancer stem cells were also up-regulated in UT2 cells, including those in the Notch, Wnt, and TGF-beta pathways. These data suggest a genetic basis for the enhanced self-renewal and tumorigenicity of osteosarcoma-initiating cell in UT2 cells. MSC, U2OS and UT2 cells were selected for RNA extraction and hybridization on Affymetrix microarrays. We sought to analysis stem/progenitor cell-associated genes and molecules involved in regulation of self-renewal signaling pathways of cancer stem cells between UT2 cells and its parent cells: U2OS (MSC works as positive control here).", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-30807", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-30807/samples/"}