ArrayExpress Uploader0humanadult pancreatic endocrine cellsnot specified3 non-selected adult human pancreasesadult pancreatic endocrine cellsnot specified3 non-selected adult human pancreasesadult pancreatic endocrine cellsnot specified4 non-selected adult human pancreasesadult pancreatic exocrine-duct cellsnot specified3 non-selected adult human pancreasesadult pancreatic exocrine-duct cellsnot specified3 non-selected adult human pancreasesnot specifiedadult brain (telencephalon)not specifiednot specifiedadult brain (telencephalon)not specifiednot specifiedadult cerebellumnot specifiednot specifiedadult cerebellumnot specifiednot specifiedadult colonnot specifiednot specifiedadult colonnot specifiednot specifiedfetal brainnot specifiednot specifiedfetal brainnot specifiednot specifiedadult kidneynot specifiednot specifiedadult kidneynot specifiednot specifiedadult livernot specifiednot specifiedadult livernot specifiednot specifiedadult lungnot specifiednot specifiedadult lungnot specifiednot specifiedadult heart musclenot specifiednot specifiedadult skeletal musclenot specifiednot specifiedadult skeletal musclenot specifiednot specifiedadult prostatenot specifiednot specifiedadult small intestinenot specifiednot specifiedadult small intestinenot specifiednot specifiedadult spleennot specifiednot specifiedadult spleennot specifiednot specifiedadult stomachnot specifiednot specifiedadult stomachnot specifiednot specifiedadult testisnot specifiednot specifiedadult testisnot specifiednot specifiedadult thymusnot specifiednot specifiedadult thymusnot specified9671arrayexpress_sid3Aims: establishment of reference samples to investigate gene expression selective for endocrine or ductal-exocrine cells within the adult...E-GEOD-30803/profile/8773/arrayexpressuploader033beta cellcellductglucagoninsulinpancreaspancreatic ductJan.17, 2015Falsegenome-wide-mrna-profiling-of-adult-human-pancreatE-GEOD-30803_A-AFFY-33Genome-wide mRNA profiling of adult human pancreatic beta and duct cells in comparison to other human tissuesJan.17, 2015Aims: establishment of reference samples to investigate gene expression selective for endocrine or ductal-exocrine cells within the adult human pancreas. To this end, human islet endocrine cells, FACS-enriched in insulin+ cells, (n=3) and human exocrine ductal cells (n=2) are compared on Affymetrix HG133A platform with duplicate hybridizations of a panel of other primary human tissues. The microarray analysis was performed on 3 pools of human beta cell-enriched cell fractions, isolated from 10 non-selected donor organs, and 2 pools of duct cell-enriched fractions obtained from 6 non-selected donor pancreases. The cells were suspension-cultured for 2-3 weeks along standard procedures and with no specific treatment prior to FACS-sorting and RNA extraction. The average composition of human beta cell-preparations was 55 ± 13% insulin+ cells, 13±8% glucagon+ cells and 21 ±7% non-granulated cells. Pancreatic duct cells-enriched preparations contained 85 ±7% cytokeratin 19+ cells with 4 ± 1% insulin+ cells and 6 ±4% glucagon+ cells and were isolated as described by Heimberg H. et al.( Diabetes 2001,49: 571-579 ). Pancreatic cell mRNA profiles were compared to those of a panel of other human primary tissues (n=2 biological replicates).http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-30803http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-30803/samples/