{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "human", "factors": [{"GSM758764 1": {}}, {"GSM758765 1": {}}, {"GSM758766 1": {}}, {"GSM758767 1": {}}, {"GSM758768 1": {}}, {"GSM758769 1": {}}, {"GSM758770 1": {}}, {"GSM758771 1": {}}, {"GSM758772 1": {}}, {"GSM758773 1": {}}, {"GSM758774 1": {}}, {"GSM758775 1": {}}], "id": 4168, "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "Homodimerization of Mpl can also be accomplished in the absence of Tpo, by binding of a synthetic ligand (Chemical inducer of...", "geo_gse_id": "E-GEOD-30585", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 0, "sample_count": 12, "tags": ["protein", "stroma"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "gene-expression-changes-induced-by-dimerization-of", "geo_id_plat": "E-GEOD-30585_A-AFFY-44", "name": "Gene expression changes induced by dimerization of intracellular c-Mpl in cord blood CD34+ progenitors", "created": "Sep.16, 2014", "summary": "Homodimerization of Mpl can also be accomplished in the absence of Tpo, by binding of a synthetic ligand (Chemical inducer of dimerization, CID) to a constitutively expressed fusion protein F36VMpl consisting of a ligand binding domain (F36V) and the intracellular signaling domain of Mpl. In contrast to Tpo stimulation, F36VMpl dimerization in human CD34+ progenitor cells generates robust erythropoiesis. Microarray gene expression profiling of progenitors demonstrated that F36VMpl dimerization, but not Tpo, results in upregulation of critical erythroid genes. CD34+ cord blood cells were transduced with F36VMpl-GFP (GFP reporter gene) and cultured on MS-5 stroma for 7 days in the presence of CID, Tpo, Epo or no factors (no CID, negative control). CD34+GFP+ cells were sorted on day 7 and subjected to microarray (n=3 independent experiments).", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-30585", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-30585/samples/"}