ArrayExpress Uploader0human4168arrayexpress_sid4Homodimerization of Mpl can also be accomplished in the absence of Tpo, by binding of a synthetic ligand (Chemical inducer of...E-GEOD-30585/profile/8773/arrayexpressuploader012proteinstromaDec.12, 2014Falsegene-expression-changes-induced-by-dimerization-ofE-GEOD-30585_A-AFFY-44Gene expression changes induced by dimerization of intracellular c-Mpl in cord blood CD34+ progenitorsSep.16, 2014Homodimerization of Mpl can also be accomplished in the absence of Tpo, by binding of a synthetic ligand (Chemical inducer of dimerization, CID) to a constitutively expressed fusion protein F36VMpl consisting of a ligand binding domain (F36V) and the intracellular signaling domain of Mpl. In contrast to Tpo stimulation, F36VMpl dimerization in human CD34+ progenitor cells generates robust erythropoiesis. Microarray gene expression profiling of progenitors demonstrated that F36VMpl dimerization, but not Tpo, results in upregulation of critical erythroid genes. CD34+ cord blood cells were transduced with F36VMpl-GFP (GFP reporter gene) and cultured on MS-5 stroma for 7 days in the presence of CID, Tpo, Epo or no factors (no CID, negative control). CD34+GFP+ cells were sorted on day 7 and subjected to microarray (n=3 independent experiments).http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-30585http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-30585/samples/