{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "human", "factors": [{"GSM756934": {"TISSUE/ORIGIN": "germ cell tumor line", "CELL TYPE": "Embryonal carcinoma cell line NCCIT"}}, {"GSM756935": {"TISSUE/ORIGIN": "normal urinary bladder", "CELL TYPE": "Normal bladder stromal cells"}}, {"GSM756936": {"TISSUE/ORIGIN": "normal urinary bladder", "CELL TYPE": "Normal bladder basal cells"}}, {"GSM756937": {"TISSUE/ORIGIN": "normal urinary bladder", "CELL TYPE": "Normal bladder urothelial cells"}}, {"GSM756938": {"TISSUE/ORIGIN": "bladder cancer", "CELL TYPE": "Bladder cancer cells"}}, {"GSM756939": {"TISSUE/ORIGIN": "bladder cancer stroma", "CELL TYPE": "Bladder cancer-associated stromal cells"}}, {"GSM756940": {"TISSUE/ORIGIN": "human stem cell XY", "CELL TYPE": "Human embryonic stem cells"}}, {"GSM75694": {"TISSUE/ORIGIN": "normal prostate", "CELL TYPE": "Normal prostate tissue"}}, {"GSM756942": {"TISSUE/ORIGIN": "prostate cancer", "CELL TYPE": "Prostate cancer tissue"}}, {"GSM75694": {"TISSUE/ORIGIN": "normal prostate", "CELL TYPE": "Normal prostate tissue"}}, {"GSM756942": {"TISSUE/ORIGIN": "prostate cancer", "CELL TYPE": "Prostate cancer tissue"}}, {"GSM75694": {"TISSUE/ORIGIN": "normal prostate", "CELL TYPE": "Normal prostate tissue"}}, {"GSM756942": {"TISSUE/ORIGIN": "prostate cancer", "CELL TYPE": "Prostate cancer tissue"}}, {"GSM756942": {"TISSUE/ORIGIN": "prostate cancer", "CELL TYPE": "Prostate cancer tissue"}}, {"GSM75694": {"TISSUE/ORIGIN": "normal prostate", "CELL TYPE": "Normal prostate tissue"}}, {"GSM756942": {"TISSUE/ORIGIN": "prostate cancer", "CELL TYPE": "Prostate cancer tissue"}}, {"GSM75694": {"TISSUE/ORIGIN": "normal prostate", "CELL TYPE": "Normal prostate tissue"}}], "id": 4163, "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "Characterization of the gene expression profiles of specific cell populations of the human urinary bladder provides an important set of...", "geo_gse_id": "E-GEOD-30522", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 2, "sample_count": 17, "tags": ["basal", "cancer", "carcinoma", "cell", "embryonal carcinoma", "lamina propria", "prostate", "stem cell", "surface", "urinary bladder"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "transcriptomes-of-human-bladder-cells-and-cells-in", "geo_id_plat": "E-GEOD-30522_A-AFFY-44", "name": "Transcriptomes of human bladder cells and cells in bladder cancer", "created": "Sep.16, 2014", "summary": "Characterization of the gene expression profiles of specific cell populations of the human urinary bladder provides an important set of research tools for the study of cellular differentiation and the cancer process. The transcriptome is a definitive identifier of each individual cell types. Surgically resected tissue was digested by collagenase and the different cell types were sorted by antibodies to cluster designation (CD) cell surface antigens. The sorted cells were analyzed by DNA microarrays. The transcriptome datasets were analyzed for differentially expressed genes and plotted on a principal components analysis space for cell lineage relationship. The following bladder cell types were analyzed: CD9+ urothelial, CD104+ basal, CD13+ stromal of lamina propria, CD9+ urothelial carcinoma cancer, and CD13+ urothelial carcinoma-associated stromal. Gene expression differences between the cell types of tumor and their respective non-cancer counterpart provide biomarker candidates. Basal cells of the bladder and prostate, although sharing CD cell surface markers, are quite different in overall gene expression. Furthermore, these cells lack transcript features of stem cell signature of embryonic stem or embryonal carcinoma cells. Cell type-specific transcriptomes are more informative than bulk tissue transcriptomes. The relatedness of different cell types can be determined by transcriptome dataset comparison. Bladder cell types were sorted from tissue specimens, and analyzed by DNA microarrays. The various transcriptomes were compared by principal components analysis for cell lineage relationship.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-30522", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-30522/samples/"}