{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSM753490": {"GENOTYPE": "kinase dead knockin  IKK\u03b1K44A/K44A"}}, {"GSM75349": {"GENOTYPE": "wild type"}}, {"GSM753490": {"GENOTYPE": "kinase dead knockin  IKK\u03b1K44A/K44A"}}, {"GSM753490": {"GENOTYPE": "kinase dead knockin  IKK\u03b1K44A/K44A"}}, {"GSM75349": {"GENOTYPE": "wild type"}}], "id": 6517, "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "IKKa, a major regulator of noncanonical and canonical NF-kB pathways, is essential for B-lymphocyte maturation and secondary lymph organ...", "geo_gse_id": "E-GEOD-30363", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 5, "tags": ["bone", "bone marrow", "cell", "liver", "lymph", "lymphocyte", "organ"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "ikk-alpha-inactivation-impairs-early-b-lymphopoies", "geo_id_plat": "E-GEOD-30363_A-AFFY-45", "name": "IKK alpha inactivation impairs early B-lymphopoiesis during hematopoiesis", "created": "Nov.11, 2014", "summary": "IKKa, a major regulator of noncanonical and canonical NF-kB pathways, is essential for B-lymphocyte maturation and secondary lymph organ formation. No evidence of IKKa regulating early B cell development currently exists. Here we found reduced pre-pro-B and pro-B cells but increased myeloid-erythroid lineages in the bone marrow (BM) of knockin mice expressing reduced and kinase-dead IKKa (KA/KA). The KA/KA BM cells recaptured their defects in wild-type recipients and KA/KA fetal liver displayed reduced B cells but increased progenitors. IKKa inactivation impaired both NF-kB pathways and deregulated expression of many genes required for early B cell commitment and hematopoiesis, including downregulated Pax5, IRF4, and Ikaros expression, but increased C/EBPa, GATA1, and Stat3 levels. Reintroduced combined NF-kB components, Pax5, and IKKa promoted BM B cell differentiation and repressed myeloid-erythroid lineages. Our studies revealed a new function of IKKa in a coordinated development process of B-lineage and erythroid-myeloid lineages during hematopoiesis via multiple pathways. Microarray analysis was performed on RNA isolated from the BM of B220+ cells isolated from 4-week old WT and KA/KA mice using affymetrix mouse 430 2.0 array chip, containing 45,000 genes, at the Laboratory of Molecular Technology SAIC-Frederick. Data were normalized, and log2 transformations were generated using Partek software (St. Louis, MO, USA).", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-30363", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-30363/samples/"}