ArrayExpress Uploader0human2D cultureRWPE-1 (immortalized prostate epithelial cells)not specified2D cultureRWPE-1 (immortalized prostate epithelial cells)not specified2D cultureRWPE-1 (immortalized prostate epithelial cells)not specified3D cultureRWPE-1 (immortalized prostate epithelial cells)2days3D cultureRWPE-1 (immortalized prostate epithelial cells)2days3D cultureRWPE-1 (immortalized prostate epithelial cells)2days3D cultureRWPE-1 (immortalized prostate epithelial cells)6days3D cultureRWPE-1 (immortalized prostate epithelial cells)6days3D cultureRWPE-1 (immortalized prostate epithelial cells)6days2D cultureLNCaP cells (prostate cancer cells)not specified2D cultureLNCaP cells (prostate cancer cells)not specified2D cultureLNCaP cells (prostate cancer cells)not specified3D cultureLNCaP cells (prostate cancer cells)2days3D cultureLNCaP cells (prostate cancer cells)2days3D cultureLNCaP cells (prostate cancer cells)2days3D cultureLNCaP cells (prostate cancer cells)6days3D cultureLNCaP cells (prostate cancer cells)6days3D cultureLNCaP cells (prostate cancer cells)6days4149arrayexpress_sid4To conduct comparative transcriptomic analyses on normal or malignant prostate epithelial cells in response to tissue contextual changes,...E-GEOD-30304/profile/8773/arrayexpressuploader318basement membranecancercellmembraneprostateprostate cancerDec.12, 2014Falsegene-expression-profiling-of-prostate-epithelial-cE-GEOD-30304_A-AFFY-44Gene expression profiling of prostate epithelial cells in response to tissue contextual changesSep.16, 2014To conduct comparative transcriptomic analyses on normal or malignant prostate epithelial cells in response to tissue contextual changes, we cultured immortalized prostate epithelial cells or prostate cancer cells as cell monolayers or three-dimensional organoids and profiled their transcriptomes in respective culture contexts. RWPE-1 (immortalized prostate epithelial cells) and LNCaP cells (prostate cancer cells) were seeded on reconstituted basement membrane (rBM)-coated culture plastics or cultured within three-dimensional (3D) rBM gels. Total RNA samples were collected from cell monolayers or 3D cell clusters (formed at day 2) or acini or spheroids (formed at day 6) in the rBM culture, followed by global gene expression profiling.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-30304http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-30304/samples/